• 제목, 요약, 키워드: soy gel

검색결과 70건 처리시간 0.041초

Effects of Specific Interaction Altering Reagents on Hardnesses of Succinylated Soy Protein Gel

  • Bae, Dongho;Jung, Hosun;Choi, Yong-Hee
    • Journal of Applied Biological Chemistry
    • /
    • v.42 no.3
    • /
    • pp.125-129
    • /
    • 1999
  • The changes in gel characteristics of soy protein and succinylated soy protein due to various specific interaction-altering reagents which affect the formation and textural properties of gels, were studied. The reagents were added to 15% soy protein solutions prior to heat treatment. Succinylated soy protein formed harder gel without the addition of reagents. Hardly no gels were formed with urea, indicating that hydrogen bonds significantly contributed to the formation and hardness of the gel and the effects of urea on the hardness of succinylated soy protein gel were more significant. Disulfide bonds were important in the formation of hard gels whether they were succinylated or not, but the contributions of hydrophobic interactions to gel hardness were relatively insignificant. The hardness reducing effects of NaCl and NaSCN were more significant in succinylated soy protein gel. As such, electrostatic interactions were important for succinylated soy protein to form hard gel but not for unmodified soy protein.

  • PDF

대두유와 슈크로오스 지방산 에스테르 첨가 녹두전분 겔의 상온 저장시의 관능적, 텍스쳐 특성 (Sensory and textural characteristics of mungbean starch gels with soy bean oil and sucrose fatty acid ester during room temperature storage)

  • 최은정;오명숙
    • 대한가정학회지
    • /
    • v.42 no.2
    • /
    • pp.213-227
    • /
    • 2004
  • This study was attempted to investigate the sensory and textural characteristics of mungbean starch gels with soy bean oil and sucrose fatty acid ester(SE) addition during room temperature storage. Freshly prepared mungbean starch gels, with and without soy bean oil and SE, were stored at 25$^{\circ}C$ for 24, 48 and 72 hours. The color value, syneresis, texture and sensory properties of the gels were measured. The lightness(L) of the gels with soy bean oil and without additives was similar whereas that with SE was lower than that without additives. Syneresis of the gels with soy bean oil and SE was lower than that without additives. Rupture stress, rupture strain and rupture energy of the freshly prepared gel with 2∼4% soy bean oil were increased, but there were no differences in rupture properties between the gel with soy bean oil and that without additives. Rupture stress, rupture stain and rupture energy of all the gels with SE were decreased. Addition of soy bean oil to the gel did not change the texture profile of the gel, whereas hardness, springiness and chewiness of the gel with SE were decreased. In sensory evaluation, the acceptability of freshly prepared gel with soy bean oil was similar to that without additives, whereas that of the gel with 2% soy bean oil stored for 24 hours was higher than that without additives. The acceptability of the gel with SE was decreased significantly.

대두단백겔의 물성에 미치는 분자결합력 저해 시약의 영향 (Effects of Various Reagents on Textural Properties of Soy Protein Gel)

  • 배동호;정호선
    • 한국식품저장유통학회지
    • /
    • v.5 no.1
    • /
    • pp.65-71
    • /
    • 1998
  • The changes in gel characteristics of soy protein as a result of various reagents that alter specific interactions which affect the formation and textural properties of gels, were studied. The reagents were added to 15% soy protein solutions prior to heat treatment. The gels were not formed with urea, indicating that hydrogen bonds significantly contributed to the formation and hardness of soy protein gel. Hydrophobic interactions and disulfide bonds compensated for hydrogen bonds and the contributions of electrostatic interactions to gel hardness are relatively insignificant. The farce primarily responsible for gel cohesiveness appeared to be disulfide bonds, because a significant decrease in cohesiveness was found only with the presence of N-ethylmaleimide. Adhesiveness decreased only with the addition of urea, and thus the contribution of hydrogen bonding to adhesiveness of gel could be concluded to be resent. However, adhesiveness was suggested to be interpreted not only wile molecular forces involved in gel formation but also with hydration properties of protein.

  • PDF

Effects of ${\beta}$-Conglycinin and Glycinin on Thermal Gelation and Gel Properties of Soy Protein

  • Kang, Il-Jun;Lee, Young-Sook
    • Food Science and Biotechnology
    • /
    • v.14 no.1
    • /
    • pp.11-15
    • /
    • 2005
  • Dynamic shear moduli of isolated soy protein solutions upon heating were measured to monitor gelation. Onsets of gelation coincide with onset temperatures of denaturation in glycinin and ${\beta}$-conglycinin solutions, whereas in isolated soy proteins, onset of gelation was above denaturation temperature of ${\beta}$-conglycinin with storage modulus increasing in two steps. The first increase in storage modulus of isolated soy proteins occurred at about $78.5^{\circ}C$, while the second increase started at about $93^{\circ}C$. Gel properties of soy protein gels having different proportions of glycinin and ${\beta}$-conglycinin were measured by compression-decompression test. ${\beta}$-conglycinin was responsible for gel elasticity. Glycinin significantly increased hardness, toughness, and fracturability of gels at high heating temperature near $100^{\circ}C$. Results reveal texture of soy protein gels can be controlled by regulating ratio of glycinin to ${\beta}$-conglycinin and heating temperature.

우무를 첨가한 콩 음료 개발 (Development of an Agar-gel Added Soy Beverage)

  • 강동수;최옥수
    • 생명과학회지
    • /
    • v.13 no.3
    • /
    • pp.258-264
    • /
    • 2003
  • 콩의 영양성과 우무의 기능성을 더하여 간편하고 편의성을 크게 증가시킨 고영양의 콩 음료 제품의 개발적성을 검토하였다. 적당한 조직감을 갖는 우무의 제조 조건으로는 1.5% 한천에 Nacl 0.1∼0.2%, glucomannan 0.1%를 첨가하는 것이 최적 조건으로 판단되었다. 그리고 콩물의 제조는 방치시 침전물의 양과 점도의 변화 등을 볼 때 고형물 10%에 설탕 3% 및 식염 0.1∼0.2% 첨가가 좋은 것으로 나타났다. 관능검사 결과 콩음료의 최적 제조조건으로는 한천 1.5%에 Nacl 0.2% 및 glucomannan 0.1%를 첨가하여 만든 우무를 고형물이 10%인 콩물에 설탕 3% 및 식염 0.1∼0.2%를 첨가하여 만든 콩물에 혼합하여 제조하는 것으로 하였다.

Development of Methods for Protein Extraction from Three Major Korean Fermented Soy Foods for 2-Dimensional Gel and Mass Spectrometric Analyses

  • Lim, Jin-Kyu
    • Journal of Applied Biological Chemistry
    • /
    • v.51 no.3
    • /
    • pp.88-94
    • /
    • 2008
  • Three different protein extraction methods-phenol extraction, trichloroacetic acid (TCA) precipitation, and desalting/TCA precipitation-were compared to determine the optimal reproducible high resolution 2-dimensional (2-D) electrophoresis for each chungkugjang, doenjang, and kochujang samples. The soluble proteins from Chungkugjang extracted by phenol were separated with high reproducibility and resolution, and gained 1.75- to 3-fold more protein spots on 2-D gel than those from the other methods. On the contrary, the extracted proteins from doenjang and kochujang treated by desalting/TCA precipitation method showed about 1.5- to 3.3-fold more protein spots on 2-D gel. Using the established methods, the changes in the protein profiles of the fermented soy foods were monitored during the fermentation period by 2-DE. One of the major proteins in soy, $\beta$-conglycinin $\alpha$-subuint, and some proteins with unknown functions were localized on 2-D gel as the protease-resistant proteins throughout the fermentation period of doenjang. Changes in the protein profile monitored by the established methods can provide basic information on unfolding the mechanisms of the generation of biofunctional activity in the fermented soy foods.

인산화와 초산화가 대두단백질의 기능특성과 구조에 미치는 영향 (Effects of Phosphorylation and Acetylation on Functional Properties and Structure of Soy Protein)

  • 김남수;권대영;남영중
    • 한국식품과학회지
    • /
    • v.20 no.5
    • /
    • pp.625-630
    • /
    • 1988
  • 대두단백질을 인산화 및 초산화처리에 의하여 변형시키고, 변형대두단백질의 기능특성을 검토하였다. 인산화 대두단백질은 비변형 대두단백질보다 높은, 용해도, 거품특성, 수분보유력을 나타냈다. 한편, 초산화에 의해서도 유화력과 거품특성이 현저하게 증진되었다. 대두단백질은 인산화와 초산화에 의하여 등전점이 산성쪽으로 변화였으며 단백질의 집괴화현상도 관찰되었다. 또한, 화학변형된 대두단백질을 디스크 전기영동에 의해 분석시 단백질밴드의 상대이동도가 현저하게 증대되었다.

  • PDF

미역과 다시마를 이용한 해조묵제조 -3. 두유혼합묵과 분리대두단백질 혼합묵- (Preparation of Seaweed Jelly(Muk) with Sea Mustard (Undaria pinnatifida) and Sea Tangle(Laminaria japonica) -3. Muks Prepared with Soy milk or Soy Protein Isolate-)

  • 정용현;국중렬;장수현;김종배;김건배;최선남;강영주
    • 한국수산과학회지
    • /
    • v.28 no.3
    • /
    • pp.325-330
    • /
    • 1995
  • 해조류 중 생산량이 많고 건강식품 소재로 가능성이 높은 미역과 다시마를 이용하여 두유와 분리대두 단백질이 첨가된 혼합묵의 제조조건을 규명하였다 두유혼합 미역묵의 제조는 두유의 비율을 증가시킴에 따라 겔강도는 감소되었고, 농축한 두유를 혼합할수록 겔강도는 증가하였다. 또한 조체용해액에 대한 두유의 비율을 7 : 1로 혼합하여 $65^{\circ}C$에서 겔화하는 것이 적절하였다. 조체용해액에 분리대두단백질을 $5\%$(w/w)를 혼합하여 $65^{\circ}C$에서 겔화할때 조체묵이나 두유혼합묵에 비해 높은 겔강도$(900g/cm^2)$을 가지는 분리대두단백질 혼합묵제조가 가능하였다

  • PDF

Anti-oxidative and Anti-inflammatory Activities of Polysaccharide isolated from Korean-Style Soy Sauce

  • Kim, Hoon;Park, Jungeun;Jung, Jaemee;Hwang, Dahyun
    • 대한의생명과학회지
    • /
    • v.26 no.1
    • /
    • pp.51-56
    • /
    • 2020
  • Soy sauce is one of the representatives of traditional fermented foods in Korea. However, studies on soy sauce are relatively insufficient in Korea compared to Japan. In this study, antioxidant and anti-inflammatory activities of polysaccharides were measured by polysaccharides isolated from two different soy sauces, Korean and Japanese (KSS-0 and JSS-0). KSS-0 was purified into two fractions using gel chromatography and named them as KSS-I and KSS-II. To investigate the antioxidant activity of the polysaccharides, we measured the polyphenol content and radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). To investigate the anti-inflammatory activity of polysaccharides, we used RAW 264.7 macrophage cells and induced inflammation using lipopolysaccharide (LPS). Then, we measured levels of inflammatory mediators such as nitric oxide (NO) and tumor necrosis factor (TNF)-α. Among the four polysaccharides, KSS-II showed the highest antioxidant activity and had good anti-inflammatory activity; KSS-II decreased inflammatory mediators in a dose-dependent manner. In conclusion, the polysaccharide isolated from Korean soy sauce (KSS-II) showed better anti-oxidant and anti-inflammatory activities than polysaccharides isolated from Japanese soy sauce, and may be useful as substances for functional foods.

대두유 첨가가 녹두전분의 이화학적 특성과 저온저장 녹두전분겔의 텍스쳐에 미치는 영향 (Physicochemical Properties of Mung Bean Starch and Texture of Cold-Stored Mung Bean Starch Gels added with Soy Bean Oil)

  • 최은정;오명숙
    • 한국식생활문화학회지
    • /
    • v.26 no.5
    • /
    • pp.513-520
    • /
    • 2011
  • This study was carried out to investigate the physicochemical properties of mung bean starch and the texture of cold-stored (5$^{\circ}C$ for 0, 24, 48, and 72 hours) mung bean starch gels added with soy bean oil (0, 2, 4, 6%). The swelling power of mung bean starch added with soy bean oil did not significantly change, whereas solubility increased significantly. Soluble carbohydrate content of mung bean starch added with soy bean oil decreased without any significant differences, whereas soluble amylose content decreased significantly. In RVA viscosity, pasting temperature and peak viscosity of mung bean starch added with soy bean oil were not significantly different, whereas minimum viscosity decreased and breakdown and consistency increased significantly. In RVA viscosity, there were no differences according to concentration of soy bean oil. DSC thermograms show that onset temperature of mung bean starch added with soy bean oil did not significantly change, whereas the enthalpy increased in the case of 4% and 6% oil addition. Rupture properties of freshly prepared mung bean starch gels added with soy bean oil increased in the case of 2% and 4% oil addition, and oil addition to mung bean starch gels suppressed changes in rupture properties during cold storage. There were no significant differences in the texture of freshly prepared mung bean starch gels added with soy bean oil, whereas hardness, chewiness, and gumminess of cold-stored mung bean starch gels added with soy bean oil decreased. In the above textural charactristics, there were no differences due to concentration of soy bean oil. Thus, the addition of 2-4% soy bean oil to mung bean starch is appropriate for improving the quality characteristics of cold-stored mung bean starch gels.