• Title, Summary, Keyword: spermiogenesis

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Spermiogenesis in the large-footed bat, Myotis macrodactylus (큰발웃수염박쥐 (Myotis macrodactylus)에 있어서의 정자변태)

  • Son, Sung-Won;Lee, Jung-Hun;Shin, Hwa-Jeung;Choi, Byung-Jin
    • Applied Microscopy
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    • v.25 no.1
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    • pp.96-110
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    • 1995
  • In order to clarify the process of spermiogenesis of the large-footed bat, Myotis macrodactylus, the testis and the epididymis obtained from mature male bats were examined by electron microscope. Based on the variety and diagnostic characters of organells, the spermiogenesis of the large-footed bat. Myotis macrodactylus could be divided into a total of nine phases. The results obtained from the present study are as follows. 1. The spermiogenesis of large-footed bat, Myotis macrodactylus was divided according to the level of fine structural differentiation into five phases, Golgi, cap, acrosome, maturation and spermiation phases, respectively; Golgi, cap, acrosome and spermiation phases were further subdivided into steps of early and late phase respectively and maturation phase has only one step. Hence, the spermiogenesis of the large-footed bat has been divided into a total of nine phases. 2. In the change of chromatin with nucleus, the chromatin granules are condensed in the whole part of nucleus at the late Golgi phase and completed at the maturation phase. 3. The sperm tail in the epididymis consists of nine outer doublets and two central singlet microtubles. Nos. 1, 5, 6, 9 of the outer dense fibers were larger than the others (2, 3, 4, 7, 8).

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Spermiogenesis in the Korean manchurian field mouse, Apodemus spesiosus peninsulae (한국산 흰넓적다리 붉은쥐 (Apodemus spesiosus peninsulae)의 정자변태)

  • Lee, Jung-Hun
    • Applied Microscopy
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    • v.26 no.2
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    • pp.221-233
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    • 1996
  • In order to study process of spermiogenesis of the Korean manchurian field mouse, Apodemus spesiosus peninsulae, the testis obtained from sexually matured male reproductive organs, were examined with electron microscopy, and the following results were obtained based on the characters of cell differentiation. 1. According to the features of cell structure, spermiogenesis of the Apodemus spesiosus peninsulae was five phases: Golgi, cap, acrosome, maturation and spermiation phase. They were further subdivided into two steps of early and late phases respectively. Hence, the spermiogenesis consists of ten steps. 2. In the changes of the chromatin, the chromatin granules began to be condenced in the cap phase and regularizated at maturation phases, and a perfect nucleus of sperm was formed at the spermiation phases. 3. The formative period of sperm tail began to be develop in the early Golgi phase and completed at the spermiation phases 4. The outer dence fibers of middle piece were arranged in a horseshoe fashion. Nos. 1, 5, 6 and 9 of the outer dense fibers were larger than the others. The structure of axoneme in the middle piece was 9+2, and the axonemal complex consists of A and B microtubules, dynein arms and radial links.

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Electron Microscopic Studies on the Spermiogenesis of the Korean Goat (한국산양의 정자완성에 관한 전자현미경적 연구)

  • 배대식;김종욱
    • Korean Journal of Animal Reproduction
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    • v.9 no.1
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    • pp.46-55
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    • 1985
  • In order to investigate the spermiogenesis of the Korean goat, the tissue obtained from the testis and the epididymis of eight matured bucks were conventionary prepared as thin sections and these sections were observed under a transmission electron microscope. Based on the development of the varous permanent organelles and certain transient structures, the spermiogenesis of the Korean goat could be divided into four main phases: Golgi, cap, acrosome and maturation; and these phases had been further subdivided into a total of nine stages. There was abundance of the contents of acrosomal vesicle contained ring-shaped material surrounding the acrosome in the early cap stage. The mitochondria made the mitochondrial sheath started from the annulus towarding the neck and formed helical arrangement in the mid maturation stage, The postacrosomal denselamina was a, pp.ared to be wave-shape in the late maturation stage. The spermiogenesis of the Korean goat was found to be basically similar to that of the Korean cattle.

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Ultrastructure of Pseudogobio esocinus (Teleostei, Cyprinidae) Spermiogenesis and Spermatozoa (모래무지(Teleostei, Cyprinidae) 정자변형과정과 정자의 미세구조)

  • Kim, Kgu-Hwan;Kwon, Deok-Mun;Lee, Jun-Ill;Jung, Sung-Bae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.3
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    • pp.147-152
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    • 2007
  • Spermiogenesis and mature spermatozoa of the Korean false minnow Pseudogobio esocinus (Cyprinidae) are described by means of scanning and transmission electron microscopy. Spermiogenesis is characterized by lateral development of the flagellum, absence of nuclear rotation, and eccentric nuclear fossa formation. The spermatozoa have a spherical head containing a nucleus with highly condensed chromatin and no acrosome. The nuclear fossa contains the proximal centriole and anterior part of the distal centriole. The midpiece is type A II and contains semi-fused mitochondria around the axoneme. However, the symmetrical distribution of 4 or more the mitochondria does not follow a general pattern of the cyprinid spermatozoa. Cytoplasmic vesicles in the midpiece are common in Cypriniformes and some of Siluriformes and Characiformes spermatozoa.

Spermiogenesis in the Korean Greater Horseshoe Bat, Rhinolophus ferrumequinum korai (한국산 관박쥐 (Rhinolophus ferrumequinum korai)에 있어서의 정자변태)

  • Lee, Jung-Hun;Choi, Byung-Jin;Son, Sung-Won
    • Applied Microscopy
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    • v.22 no.2
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    • pp.97-117
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    • 1992
  • In order to study process of spermiogenesis of the Korean greater horseshoe bat, Rhinolophus ferrumequinum korai, the cycle of seminiferous epithelium was examined by the light and electron microscope and the following results were obtained based on the epithelial cell differentiation. 1. Spermiogenesis occurred from early July to mid-Octber, and spermatogenic activity was vigorous from mid-August to late September. Spermatocytes including spermatogonia were found to be degenerated in only July. It is deduced that the degeneration serves as the mechanism to regulate effective use of energy to prepare for mating and hibernating periods, and regulation of breeding cycle. 2. Spermiogenesis of the Korean greater horseshoe bat was divided according to differentiation of the cell structure, into Golgi, cap, acrosome, maturation and spermiation phases; Golgi, cap and spermiation phases were further divided into two steps of early and late phase respectively, and acrosome phase into three steps of early, mid and late phases, and maturation phase has only one step. Hence, the spermiogenesis consists of ten phases. The first research was done in this article on the changes of chromatin with nucleus, the time of appearance and disappearance of chromatin granules, in case of Korean greater horseshoe bat (Rhinolophus ferrumequinum korai). Chromatin granule began to be condensed in late Golgi and the condensation proceeded to form an irregular mass of a electron-dense chromatin in a form of circular cylinder in the center of nucleus at the phase of maturation. Finally, the chromatin condensation proceeded and perfect nucleus of sperm with homogeneous density was formed when the sperm was separated from Sertoli cell. Therefore, appearance and disappearance of chromatin granules occurred in the period of time between late Golgi and maturation phase, The tail of sperm began to develop in early cap phase, Numerous lipid droplets were obseved in the cytoplasm of spermatids during the maturation phase, which seemed to be used as energy source necessary to make mature sperm during spermiogenesis.

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Differentiation of Seminiferous Epithelium and Spermiogenesis in the Testis of Rana catesbeiana (황소개구리(Rana catesbeiana)의 세정관 상피의 분화와 정자변태)

  • Go, Song-Haang;Lee, Jung-Hun
    • Applied Microscopy
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    • v.31 no.2
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    • pp.143-156
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    • 2001
  • The aim of this study was to clarify the differentiation of seminiferous epithelial cells and spermiogenesis in the testis of Rana catesbeiana. Spermatogenesis of R. catesbeiana consists of primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids. They were subdivided into eight stages on the basis of the morphological features of the germ cell differentiation. From the spermatocytes except primary spermatogonia to before the spermiation of spermatids were surrounded by spermatocyst. Spermiogenesis of R. catesbeiana can also be divided into three stages on the basis of morphological features of the nucleus and the cytoplasm organelles. Spermatozoon contained a saccular acrosome, a cylindrical and tapered slighty at both ends head, and a tail with only the axoneme.

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Anatomical Ultrastructure of Spermiogenesis and Spermatozoa of Pseudobagrus fulvidraco (Siluriformes: Bagridae) from Korea (한국산 동자개(Pseudobagrus fulvidraco)의 정세포변형과정과 성숙한 정자의 미세해부학적 구조(Siluriformes: Bagridae))

  • Kim, Kgu-Hwan;Kwon, Deok-Mun
    • Korean Journal of Ichthyology
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    • v.21 no.2
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    • pp.81-86
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    • 2009
  • The spermiogenesis and mature spermatozoa of Pseudobagrus fulvidraco were described by means of scanning and transmission electron microscopy. Spermiogenesis is characterized by lateral development of the flagellum, nuclear rotation, deep nuclear fossa formation and compaction into thick granules. The spermatozoa exhibit a round head containing a nucleus that lacks an acrosome, and having a midpiece and a flagellum. The midpiece is small and has a short cytoplasm including several mitochondria separated from the tail by the cytoplasmic canal. The flagellum contains the 9+2 classical axoneme structure and has two axonemal fins. The presence of axonemal fins in the flagellum is a common character in Bagridae. The interrelationships among the Bagridae as well as other teleosts are herein discussed.

Spermiogenesis in the Korean Daubenton's Bat(Myotis daubentonii ussuriensis) (한국산 물윗수염박쥐(Myotis daubentonii ussuriensis)의 정자변태)

  • 손성원
    • Development and Reproduction
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    • v.1 no.1
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    • pp.9-24
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    • 1997
  • To investigate the process of spermiogenesis of the Korean eastern Daubenton's bat, Myotis daubentonii ussuriensis, the testis obtained from mature male bats was studied by transmission electron microscope and were based on the variety and diagnostic characters of cell organells. The results obtained from the present study are as follows. According to the differentiation of the cell organells, the spermiogenesis of the Korean eastern Daubenton's bat, M. d. ussuriensis, was divided into Golg, cap, acrosome, maturation and spermiation phases. Besides, these Golgi, cap, acrosome, and maturation phase were subdivided into the steps of early and late phases repectively and matruation phase was subdivided into step of early, mid and late phases. Therfore, the spermiogenesisof M. d. ussuriensis has been divided into a total of 11 phases. The chromatin granules began to condense at the early cap phase, regularized at the acrosome phase, and a perfect nucleus of sperm was formed at the maturation phase. The chromatoid body was occurred in the upper cytoplasm of nucleus at the early Golgi phase, and it was accurred the posterior cytoplasm of the nucleus at the early maturatio phase. The formation of sperm tail began to be develop in the early golgi phase, and completed at the spermiation phase. The fiber structure of middle piece was consisted of nine outer doublets and two central singlet microtubules and Nos. 1, 5, 6 and 9 in the outer dense were larger than the others(2, 3, 4, 7, 8).

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Spermiogenesis in the Saghalien Pygmy Shrew, Sorex minutus gracillimus (쇠뒤쥐 (Sorex minutus gracillimus)의 정자변태)

  • Heo, Jin-Chol;Lee, Jung-Hun
    • Applied Microscopy
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    • v.31 no.2
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    • pp.129-141
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    • 2001
  • To investigate the spermiogenesis of the Saghalien Pygmy shrew (Sorex minutus gracillimus), the testis obtained from mature male shrew was studied by electron microscopy, and the following results obtained based on the morphological characteristics of cell differentiation of the seminiferous epithelium in the testis. According to the fine structural differentiation, spermiogenesis of S. minutus gracillimks was divided into Golgi, cap, acrosome, maturation and spermiation phases. Beside, the Golgi and cap phases were subdivided into three steps of early, middle and late phase respectively, and acrosome phase into two steps of early and late phase , and maturation and spermiation phases has only one step respectively. Thus, the spermiogenesis of S. minutus gracillimus was divided into a total of ten steps. The chromatin granules begin to be condensed in the acrosome phase, and a perfect nucleus of sperm was formed at the spermiation phase. Mancette were appeared from the late acrosome phase to the maturation phase. The formation of sperm tail began to develop in the late Golgi phase, and completed at the spermiation phase. Multivesicular bodies were appeared from the Golgi phase to the maturation phase, recognized with pale, pale and moderate, and dense at Golgi, cap and acrosomal and matulation phases respectively.

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Spermiogenesis and Spermatozoal Ultrastructure of the Roundnose Flounder, Eopsetta grigorjewi (Teleostei: Pleuronectidae) (물가자미 (Eopsetta grigorjewi)의 정자변태 및 정자 미세구조)

  • AN Cheul Min;LEE Jung Sick;HUH Sung-Hoi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.6
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    • pp.730-736
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    • 1999
  • The roundnose flounder's (Eopsetta grigorjewi) spermiogenesis and fine structure of the spermatozoon were examined by means of the scanning and transmission electron microscopy, During the spermiogenesis, the chromatin of the spermatid became fine granular form, and progressively condensed into many large globules, finally homogeneously condensed in the spermatozoan head. The main characteristics of the spermiogenesis were the disappearanre of Golgi complex, the appearance of microfilament the reduction of mitochondria and the appearance of Iysosome in the cytoplasm. A spermatozoon consisted of head and tail, but the acrosome was absent. The cytoplasmic collar containing seven mitochondria was observed in the posterior part of the head. The well-developed axonemal lateral fins were observed in the tail. The cross section of the axial filament showed '9+2' axonemal structure of microtubules, and the numerous vesicles were observed in the cytoplasm.

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