• Title/Summary/Keyword: sugar cane bagasse

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Solid Substrate and Submerged Culture Fermentation of Sugar Cane Bagasse for the Production of cellulase and Reducing Sugars by a Local Isolate, Aspergillus terreus SUK-1

  • Wan Mohtar, Yusoff;Massadeh, Muhannad Illayan;Kader, Jalil
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.770-775
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    • 2000
  • Several process parameters were studied to ascertain the effect on degradation of sugar cane bagasse in relation to the production of cellulase enzyme and reducing sugars by Solid Substrate Fermentation (SSF) and Submerged Culture Fermentation (SCF) of Aspergillus terreus SUK-1. The effect of air-flow rate (0-1.3 v/v/m), of different ratios of substrate weight to liquid volume (1:6, 1:10, 1:20, and 1:30 w/v, g/ml), scale-up effect (10, 20, and 100 times of 1:10 ration, w/v) and the effect of temperature (30, 40, 50, and $60^{\circ}C$) in SSF were studied. Air-flow rate of 1.0 v/v/m gave the highest enzyme activity (FPase 0.25 IU/ml, CMCase 1.24 IU/ml) and reducing sugars concentration (0.72 mg/ml). Experiment using 1:10 ratio (w/v) was found to support maximum cellulase activity (FPase 0.58 IU/ml, CMCase 1.97 IU/ml) and reducing sugar concentration (1.23 mg/ml). Scaling-up the ratio of 1:10(w/v) by a factor of 20 gave the highest cellulase activity (FPase 0.71 IU/ml, CMCase 2.25 IU/ml) and reducing sugar concentration (3.67 mg/ml). The optimum temperature for cellulase activity and reducing sugar production was $50^{\circ}C$(FPase 0.792 IU/ml, CMCase 2.25 IU/ml and 3.85 mg/ml for reducing sugar concentration). For SCF, the activity of cellulase enzyme and reducing sugar concentration was found to be lower than that obtained for SSF. The highest cellulase activity obtained in SCF was 50% lower than the highest cellulase activity in SSF, while for reducing sugar concentration, the highest concentration obtained in SCF was 90% lower than that obtained in SSF.

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BIOLOGICAL ACTIVITIES OF PRODUCTS FROM SUGAR CANE BAGASSE FERMENTATION BY Pleurotus sajor-caju (사탕수수 찌꺼기를 이용한 여름느타리 발효부산물의 생물활성)

  • Lee, Young-Keun;Chang, Hwa-Hyoung;Kim, Won-Rok;Kim, Jin-Kyu;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.39-42
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    • 1998
  • In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively determined.

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Studies on the Comparative Analysis of Immunofunction of Agaricus blazei Murill Cultivated with Fermented Media Containing Pueraria thunbergiana (칡혼합 발효배지로 생산된 신령버섯의 면역기능성 비교 분석에 관한 연구)

  • 김주남;서정식;박동철
    • Korean Journal of Food Preservation
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    • v.9 no.1
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    • pp.114-119
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    • 2002
  • This research was performed to investigate the immnomodulative effects of ploysaccharides extracted from the fruiting body of Agarcus blazei cultivated with the media which are fermented with sugar cane bagasse containing Pueraria thunbergiana in open-air storage. In MTT test, methanol extracts from the fruiting body of A. blazei cultivated with P. thunbergiana media showed in colon carcinoma line(HT29) by 1.5∼3.5 fold and human heptoma cell line (HepG2) by 1.3 ∼2.4 fold antitumor activites compared to two types media (rice straw plus sugar cane bagasse, rice straw only) often used in the fauns. To clarify the antimutagenic principles, three extracts, Ab-l, Ab-2 and Ab-3, were separated by the solvent fractionations such as hot water, cold & hot sodium hydroxide respectively, and their antimutagenic effects was determined against N-methyl-N'-nitro-N-cnitrso-guanidine(MNNG) using Salmonella typhymurium. There was no significant differencies of inhibition levels among the used media, but Ab-3 tractions still showed a high antimutagenicity in the Ames test regardless of cultivating areas or media. To prove the cell immunofunction, nitric oxide (NO) produced from Raw 264.7 matrophage cultured with three fractions (Ab-l, Ab-2, Ab-3) was measured, and showed generally increase about 45 ∼58 percent compared to another two media (rice straw plus sugar cane bagasse, rice straw only), in the fraction of hot alklai extracts of the fruiting body cultivated with P. thunbergiana, which means that the media selection could be very important factors for improving medicinal effects in agaricus blazei fruiting body.

Synthesis of Zeolites ZSM-5 and ZSM-48 from Gasification Ashes of Agricultural Wastes

  • Lin, Kuen-Song;Lin, Wen-Chiang;Chitsan Lin
    • Proceedings of the IEEK Conference
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    • pp.610-615
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    • 2001
  • Over 800 thousand tons per year (TPY) agricultural biowastes, such as sugar cane bagasse, sugarcane leaf, rice straw, rice husk and corn leaf, are produced in Taiwan. These biomasses are the major types of agricultural wastes and are abundantly available. However, these biowastes cause disposal and landfill problems. Ossification ashes of the agricultural biowastes containing 70-95 % amorphous silica would make the utilization system of agricultural biowaste ashes become highly economically and environmentally attractive. Experimentally, high crystallinity (99%$^{+}$) zeolites ZSM-5 and ZSM-48 synthesized from the reaction mixtures containing a silica source from ashes of these biowastes gasification were investigated. Tetrapropylammonium bromide (TPABr) and 1,6-diamino-hexane (C$_{6}$ DN) were used as structure-directing agents in syntheses of ZSM-5 and ZSM-48, respectively. X-ray powder diffraction (XRD) and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDX) data indicated that ZSM-5 or ZSM-48 with a high crystallinity can be obtained within 48 hours of crystallization in the high pressure (15-20 atm) autoclave at 393-473 K. The Si/Al ratios of synthetic zeolite products were determined by X-ray fluorescence (XRF) and induced couple plasma/mass spectroscopy (ICP/MS). It was observed that the ZSM-5 crystals a.e composed of hexagonal rod-shaped crystals with typically 8-13 пm in size by SEM. In addition, ZSM-48 crystalline materials are composed of spherical aggregates of needle-shaped or rod-like crystals with typically 2-3 пm in diameter and 6-8 пm in length.h.

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Effect of Dietary Fiber Sources on Chewing Activity in Sheep (섬유소 공급원이 면양의 저작행동에 미치는 영향)

  • Kim, M.H.;Lee, S.R.;Seo, I.J.;Maeng, W.J.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.639-646
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    • 2007
  • To evaluate the effect of fiber sources on chewing activity, five sheep were consecutively fed diets containing 45% of a fiber source selected from 7 tested fiber sources of alfalfa hay cube(AHC), corn cob (CC), corn silage(CS), cotton seed hull(CSH), peanut hull(PHL), rice straw(RS) and sugarcane bagasse (SCB). Number of chew showed significantly higher value in CC(p<0.001) then other sources. RS and CC had highest(p<0.01) rumination times of 352 and 367 min/d, respectively. CC also showed the highest number of chew per kg NDF intake(p<0.01), but rumination time per kg NDF intake showed no difference except CSH(p<0.001). These results suggest that chewing activities were greatly affected by the fiber sources, and therefore it should be accounted in the formulation for ruminant feed.

Effects of Fiber Sources on Ruminal pH, Buffering Capacity and Digestibility in Sheep (섬유소 공급원이 면양의 반추위 pH, Buffering Capacity 및 소화율에 미치는 영향)

  • Seo, In-Jun;Kim, Myeong-Hwa;Kim, Dong-Sik;Lee, Sang-Rak;Maeng, Won-Jai
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.3
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    • pp.177-184
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    • 2005
  • This study was conducted to study the effect of fiber sources on ruminal pH and buffering capacity and whole digestive tract digestibility with five ruminally fistulated sheep. Evaluated fiber sources were alfalfa hay cube (AHC), corn cob (CC), com silage (CS), cotton seed hull (CSH), peanut hull (PHL), rice straw (RS), and sugarcane bagasse (SCB). Sheep were fed consecutively a diet containing each tested fiber source $(45\%)$ with a corn-based concentrate diets $(55\%)$ during each experimental period. Ruminal pH showed no difference among fiber sources except the significantly lower pH at 8h (p<0.05) with RS (pH; 5.78) than those in other sources. Buffering capacity showed significant differences at 0h (p<0.05) and 2h (p<0.05) after feeding in CS compared to those from SCB and CC, while there was higher in PHL (p<0.01) at 12h post feeding except AHC than CC, CS, SCB, and RS. Dry matter digestibility was significantly higher in CS (p<0.001) than in other sources. Neutral detergent fiber digestibility was higher in CC, CS, CSH, and RS than other fiber sources (p<0.001). These results suggest that ruminal pH and buffering capacity and whole digestive track digestibility were significantly affected by fiber sources in ruminant diet, and, therefore, should be took those effects into account for TMR formulation.