• Title, Summary, Keyword: suppressive subtraction

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Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng

  • Kim, Doo-Young;Kwon, Ki-Rok;Kang, Won-Mo;Jeon, Eun-Yi;Jang, Jun-Hyeog
    • Journal of Pharmacopuncture
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    • v.15 no.1
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    • pp.18-22
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    • 2012
  • Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.

Identification and Analysis of the Chloroplast rpoC1 Gene Differentially Expressed in Wild Ginseng

  • Lee, Kwang-Ho;Kwon, Ki-Rok;Kang, Won-Mo;Jeon, Eun-Mi;Jang, Jun-Hyeog
    • Journal of Pharmacopuncture
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    • v.15 no.2
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    • pp.20-23
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    • 2012
  • Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with microarrays, realtime polymerase chain reaction (PCR), and reverse transcription PCRs (RT-PCRs). One of the clones isolated in this research was the chloroplast rpoC1 gene, a ${\beta}$subunit of RNA polymerase. Real-time RT-PCR results showed that the expression of the rpoC1 gene was significantly upregulated in wild ginseng as compared to cultivated ginseng, so, we conclude that the rpoC1 gene may be one of the important markers of wild ginseng.

Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng

  • Han, Young-Ju;Kwon, Ki-Rok;Kang, Won-Mo;Jeon, Eun-Yi;Jang, Jun-Hyeog
    • Journal of Pharmacopuncture
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    • v.16 no.1
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    • pp.30-36
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    • 2013
  • Objective: Panax ginseng is one of the most medicinally used herbal medicines in the world. Wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the differences between wild ginseng and cultivated ginseng. Method: To identify wild ginseng-specific genes, we used suppressive subtraction hybridization. Results: We report that one of the clones isolated in this screen was the GAPDH (glyceraldehyde 3-phosphate dehydrogenase) gene (designated pGAPDH-w). DNA BLAST sequence analysis revealed that this pGAPDH-w gene contained novel sequences of 94 bp. RT-PCR results showed that the expression of the pGAPDH-w gene was significantly up-regulated in the wild ginseng as compared with the cultivated ginseng. Conclusion: The pGAPDH-w gene may be one of the important markers of wild ginseng.