• Title, Summary, Keyword: swine toxoplasma

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On the distribution of Toxoplasma antibodies in Cheju-Do 1. Distribution of Toxoplasma antibodies in swine, cats and butchers (제주도에(濟州道) 있어서 Toxoplasma 항체분포(抗體分布)에 관(關)한 연구(硏究) 1. 돼지, 고양이 및 식육취급자(食肉取扱者)에 있어서의 Toxoplasma 항체분포(抗體分布)에 대하여)

  • Kim, Seung-ho;Kim, Young-ju
    • Korean Journal of Veterinary Research
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    • v.29 no.3
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    • pp.333-342
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    • 1989
  • Effect of raising types and environmental conditions on the infection of Toxoplasma in the swine, the cat and the man were studied in Cheju Island from Sept. 1987 to Aug. 1988. Blood samples were taken from 214 conventionally raised swine in 6 villages and 506 swine raised in swine specialized farms, 122 cats raised under free moving or restraned conditions in 8 locations, 113 butchers, and 210 villagers. Toxoplasma antibody values of the blood sera were determined using the enzymelinked immunosorbent assay (ELISA). The eating type of viscera was also investigated by using questionaires 1. When ELISA method was used, the percentage of Toxoplasma infected swine among the conventionally raised and of those raised in swine specialized farms were 60.7% and 21. 390, respectively. The respective mean of antibody values (${\pm}SD$) were 0.589 (${\pm}0.310$) and 0.385 (${\pm}0.237$) and differed very significantly (p<0.01). A significant difference was also found in antibody values among 6 villages (p<0.05). 2. The mean infection percentage of toxoplasma in the cat was 38.2%. the infection percentage for cats raised under free-moving and re~;trained condition were 37.0% and 38.2% respectively. The respective antibody values(${\pm}SD$) for toxoplasma were 0.600(${\pm}0.614$) and 0.637 (0.645), and did not difference significantly. 3. The infection percentage of toxoplasma in villagers and butchers were 26.2 and 38.3% respectively. The respective antibody values (SD) for toxoplasma were 0.429(${\pm}0.195$) and 0.341 (${\pm}0.236$), and differed very significantly (p<0.01). There were also highly significant differences Pyo-sun and other village (p<0.01). 4. Analysis of the questionaires showed that 26.0% of 392 villages eated liver and some villagers eated other viscera.

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Survey on the Distrributions of Swine Toxoplasma Antibodies by Latex Agglutination Test in Gyeongnam Central Area (경남 중부지역에서의 Latex응집반응을 이용한 돼지 톡스플라즈마병 항체분포 조사)

  • 이병훈;황보훈;변유성;이순선;김차용;서명득
    • Korean Journal of Veterinary Service
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    • v.15 no.2
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    • pp.174-183
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    • 1992
  • This study was conducted to determine the serum antibodies against toxoplasma in swine from breeding-pig farm, pig farm and abattoir by latex agglutination(LA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical co.). The results obtained were summerized as follows : 1. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum dilution of 1 ; 32. 2. positive rates of toxoplasma antibodies in 823 swine sera were 17.0%(140 cases) by LA test. 3. The toxoplasma antibody detection rates against 194 swine sera in breeding-pig farm, 273 swine sera in pig farm and 356 swine in abattoir were 46.9%(91 cases), 8.4%(23 cases) and 7.3% (26 cases) , respectively. 4. In LA test serum antibody titers in 823 test sera were shown as 51 cases (36.4%) in 1 : 32, 40(28.6%) in 1;64, 17(12.1%) in 1:128, 14(10.0%) in 1:256, 10(7.1%) in 1:512, 5(3.6%) in 1:1,024, and 3(2.1%) in 1 : 2,048. 5. Positive rates of toxoplasma antibodies in swine sera from each breeding-pig farm were 20.0∼61.9%.

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Studies on the Toxoplama gondii in Buffy Coat Cell Culture I. Multiplication of Toxoplasma gondii in Swine Origin Buffy Coat Cells (Toxoplasma gondii의 조직배양(組織培養)에 관한 연구(硏究) I 돼지의 Buffy Coat Cell Culture에서의 증식소견(增植所見))

  • Kim, Jeong Myeon
    • Korean Journal of Veterinary Research
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    • v.13 no.1
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    • pp.67-73
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    • 1973
  • Toxoplasma gondii (Tp), RH strain, was inoculated into cultured buffy coat cells obtained from the swine blood. The main reason for adopting swine lies in the animal's unusual susceptibility to Tp, As for the culture method used in the experiment, those well proved methods practised by Cho, Merchant, Moore and Tarnvik were mainly referred to as a starting point: hence, the author's method has been turned out to be the modified or supplementary form of those methods. Observations were made on the phase of multiplication of Tp in the cytoplasm. The results obtained were as follows: 1. Better growth and multiplication of Toxoplasma gondii were noticeably observed in the swine buffy coat cell, inoculated after three-to-five day cultivation of the cell. 2. In the lapse of the observation period, there appeard Toxoplasma gondii rarely available in the earlier stage, which had been inoculated into the cell after three-to-five day cultivation. In other words, Toxoplasma gondii started to show itself in seven or eight hours after inoculation, most outstandingly noticeable between twenty four hours and forty eight hours. Thereafter the disintegration stage of Toxoplasma gondii was observed.

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Use of the enzyme-linked immunosorbent assay for the detection of toxoplasmosis in swine (ELISA를 이용한 돼지 톡소플라스마병의 조기 진단에 관한 연구)

  • Suh, Myung-deuk;Jang, Dong-hwa;Joo, Hoo-don
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.567-575
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    • 1989
  • This study was conducted to evaluate the possibility of application of a microenzyme-linked immunosorbent assay(micro-ELISA) for the serodiagnosis of specific toxoplasma antibodies in swine sera and this test was performed as a microplate system by coating the polystyrene plates with toxoplasma soluble antigen, incubated serially diluted sera, then added horse radish peroxidase labelled goat anti-swine IgG(r) conjugate followed by o-phenylenediamine as substrate. The color development by enzyme-substrate reaction was determined by the photometric reading [ELISA reader at 490nm (OD)] and visual reading. The soluble antigen was prepared from the tachyzoites in mouse peritoneal cavity. A total of 1,200 swine sera from pig slaughter-house and a total of 116 swine sera from pig breeding station (S-C farm) were tested for the detection of antibodies to Toxoplasma gondii. The results obtained were summarized as follows: 1. The optimal reactions of indirect ELISA for the test sera were determined by the dilution of antigen 1:256 and 1:3,200 of horse radish peroxidase conjugate [anti-swine IgG(r)]. 2. The specific togoplasma antibody(IgG) in pigs infected with Tp artificially were detected as the serum titers of 1:64 or 1:128 at one week postinfection. 3. Of a total of 1,200 swine sera from pig slaughter-house 505 samples of sera were detected as positive (42.1%) and of a total of 116 swine sera from S-C pig breeding station 68 samples of sera as positive (58.6%). 4. The specific antibody(IgG) detection rates against a total of 1,200 test sera from pig slaughter-house were not significant between male (43.1%) and female (40.7%). 5. The indirect ELISA was proved to be a sensitive and specific procedure for the serodiagnosis of swine toxoplasmosis and also evaluated as an effective screening test for the large scale of test samples in laboratory.

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Prevalence of toxoplasma antibody in swine by microplate latex agglutination test in Kyeongnam district (Latex 응집반응에 의한 경남중부지역 돼지의 톡소플라즈마병 항체조사)

  • Lee, Byung-hoon;Kim, Cha-yong;Suh, Myung-deuk
    • Korean Journal of Veterinary Research
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    • v.33 no.2
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    • pp.263-268
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    • 1993
  • This study was conducted to detect the serum antibody of toxoplasma in swine from breeding-pig, rearing-pig farm and slaughtered pig in abattior by latex agglutination(LA) test. The perfomance of LA test was carried out with commercial Toxo-MT kit(Eiken Chemical Co.)by Tsubota and Ozawa's method. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum titer of 1 : 32. Positive rate of toxoplasma antibody from the total of 823 serum samples by LA test was 17.0%(140 cases). And positive rates of toxoplasma antibody against serum samples of 194 from breeding-pig farm, 273 from rearing-pig farm and 356 from abattior were 91 cases(46. 9%), 23 cases(8.4%) and 26 cases(7.3%), respectively. The distributions of serum antibody titers in 823 test sera by LA test were shown 51 cases(36.3%) in 1:32, 40(28.6%) in 1:64, 17(12.1%) in 1:128, 14(10.0%) in 1:256, 3(2.1%) in 1:512, 5(3.6%) in 1:1024 and 3(2.1%) in 1:2048. The ranges of positive rate from the sera in each group of breeding-pig farms were 20~61.9%.

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Incidence of Antibodies for Toxoplasmosis of Cattle and Pigs in Jeonnam District (전남지역에서 사육하는 소와 돼지의 Toxoplasma 항체 조사성적)

  • Seo Doo-Seok;Kim Oh-Nam;Kang Byung-Kyu
    • Journal of the korean veterinary medical association
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    • v.16 no.1
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    • pp.29-34
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    • 1980
  • The latent infection of Toxoplasma in swine, Holstein cow and Korean native cattle raised in Jeonnam prefacture during 1978 was proved serologically. Of the 127 swine, 73($57.4\%$) cases were positive for the indirect hemagglutination test, and

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Studies on Toxoplasmosis. - II. Serological Survey of Toxoplasmosis on Swine by Complement Fixation Inhibition Test. (Toxoplasmosis에 관한 연구 - 제2보:보체결합저지반응에 의한 돈 Toxoplasmosis의 분포조사.)

  • Mun Jae Bong
    • Journal of the korean veterinary medical association
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    • v.9 no.2
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    • pp.23-34
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    • 1965
  • In 1957, a Toxoplasma infection among swine was first discovered in Korea. Thereafter, Complement Fixation Inhibition Test was applied to 2,017 swine serum samples which were obtained from all over the country except Cheju Island. Significant results are

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