• Title, Summary, Keyword: thiodicarb

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Insecticidal activity of thiodicarb on lepidopterous pests (나방류에 대한 thiodicarb의 살충활성)

  • Choi, Yu-Mi;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.8 no.1
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    • pp.16-21
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    • 2004
  • A series of experiments was conducted to determine the toxicities of thiodicarb on the six lepidopterous pests (Pseudaletia separata, Plutella xylostella, Palpita indica, Spodoptera exigua, Helicoverpa assulta, Spodoptera litura) and to elucidate factors insecticidal effects mechanism of thiodicarb. Thiodicarb was very effective against six lepidopterous young larvae, but less effective to the old larvae and it acted slowly. Thiodicarb inhibited acetylcholinesterase and glutathione S-transferase activities, but not inhibit esterase activity.

Residue analysis of insecticide thiodicarb in sweet persimmon and its safety evaluation (살충제 thiodicarb의 단감 중 잔류분석 및 안전성 평가)

  • Lo, Seog-Cho;Hwang, Cheol-Hwan;Kim, Min-Soo;Ma, Sang-Yong;Han, Seong-Soo
    • The Korean Journal of Pesticide Science
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    • v.8 no.3
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    • pp.184-188
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    • 2004
  • The residue analysis of the insecticide thiodicarb in sweet persimmon was analyzed with a gas chromatograph equipped with nitrogen phosphorus detector(NPD) to evaluate efficacy of analysis method and safety of thiodicarb in persimmon. Minimum detectable amount of methomyl oxime, hydrolysate of thiodicarb, was 0.2 ng and detection limit of thiodicarb was 0.05 ppm. The mean recoveries evaluated from untreated samples spiked at 0.5 ppm and 2.5 ppm were $93.9{\pm}3.9$ and $92.8{\pm}2.0%$, respectively. When thiodicarb was sprayed onto the sweet persimmon two times until 40 and 30 days before harvest, three times until 40, 30 and 21 days before harvest, and four times until 40, 30, 21 and 14 days before harvesting, the mean residual amount in sweet persimmon were $1.50{\pm}0.04$, $1.86{\pm}0.04$ and $2.11{\pm}0.12$ ppm, respectively. It would be safe when thiodicarb 40% WP is treated four times 14 days before harvesting to control fruit moth in sweet persimmon.

Selection of Insecticides for Controlling Chestnut Curculio (Curculio sikkimensis) (밤바구미 방제를 위한 살충제의 탐색)

  • Kim, Young-Jae;Han, Jong-Been;Seo, Dong-Kyu;Kim, Yeon-Tae;Park, Byeong-Kyu;Choi, Kwang-Sik;Kim, Chul-Su;Shin, Sang-Chul;Lee, Sang-Gil;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.8 no.4
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    • pp.347-352
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    • 2004
  • Susceptibility of Curculio sikkimensis adults to 39 commercial insecticides were evaluated by body dipping method, Among them sixteen insecticides of chlorpyrifos, fenitrothion, fiponil, phenthoate, benfuracarb, thiodicaib, carbosulfan, bifenthrin, cypermethrin, $\lambda$-cyhalothrin, deltamethrin, clothianidin, acetamiprid+diflubenzuron, etofenpox+diazinon, esfenvalerate+fenitrothion and furathiocarb +diflubenzuron showed 100% mortality. Insecticides with over 80% residual effect at 1 day after treatment were phenthoate, benfuracarb and thiodicarb (95.0, 82.5, and 85.0%, respectively). However, after that, it was dropped rapidly. Other insecticides showed low residual effect. In the control efficacy test on C. sikkimensis, insecticides with over 90% control values at 1 day after treatment were phenthoate, thiodicarb, fipronil, acetamiprid+bifenthrin and furathiocarb+diflubenzuron. However, all insecticides from 2 days after treatment were over 90%.

Influence of plant surface spray adhesion of dinotefuran and thiodicarb on control of apple leafminer

  • Kim, Young-Shin;Kim, Kwang-Soo;Jin, Na-Young;Yu, Yong-Man;Youn, Young-Nam;Lim, Chi-Hwan
    • Korean Journal of Agricultural Science
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    • v.43 no.3
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    • pp.346-352
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    • 2016
  • This study was conducted to obtain the correlation between the plant surface spray adhesion amount of pesticides and the pest control effect. The linearity of the standard curves of dinotefuran and thiodicarb was $R^2=0.9999$, and recovery was between 70% to 120% which was satisfactory for insecticide residue analyses. The pest control effect was evaluated by assessing the number of apple leafminers (Phyllonorycter ringoniella, Gracillariidae, Lepidoptera) captured by sex pheromone traps from late June to late September in 2015. For the adhesion amount, dinotefuran recovered from trap A and B, respectively were $47{\mu}g/50cm^2$ and $23{\mu}g/50cm^2$, which can be characterized as a very low adhesion amount in comparison to the average adhesion amount of $81{\mu}g/50cm^2$ in the field. In case of thiodicarb, $691{\mu}g/50cm^2$ and $71{\mu}g/50cm^2$ were recovered from trap A and B, respectively, and the average amount in the field is $325{\mu}g/50cm^2$. These results showed close correlation with the insect population captured by trap A and B. The numbers of insects captured by trap A and B between the end of July and late August were similar. After spraying thiodicarb on August 28, the number of apple leafminers captured by trap B is bigger than that of trap A. It appears that pest occurrence tended to be high at low adhesion amounts of the active ingredient. Therefore, in order to obtain an optimal control effect, it is suggested that uniform application of insecticides is critical instead of relying on the amount of insecticide applied in the field.

Detection of Multi-class Pesticide Residues Using Surface Plasmon Resonance Based on Polyclonal Antibody

  • Yang, Gil-Mo;Kang, Suk-Won
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.547-552
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    • 2008
  • The detection of carbamate (carbofuran, carbaryl, benfracarb, thiodicarb, and methomil) and organophosphate (diazinon, cadusafos, ethoprofos, parathion-methyl, and chlorpyrifos) pesticide residues with very low detection limits was carried out using surface plasmon resonance (SPR) based equipment. The capacity to develop a portable SPR biosensor for food safety was also investigated. The applied ligand for the immunoassays was polyclonal goat anti-rabbit immunoglobulin (IgG) peroxidase conjugate. Concentration tests using direct binding assays showed the possibility of quantitative analysis. For ligand fishing to find a proper antibody to respond to each pesticide, acetylcholinesterase (AChE), and glutathione-S-transferase (GST) were tested. The reproducibility and precision of SPR measurements were evaluated. With this approach, the limit of detection for pesticide residues was 1 ng/mL and analysis took less than 11 min. Thus, it was demonstrated that detecting multi-class pesticide residues using SPR and IgG antibodies provides enough sensitivity and speed for use in portable SPR biosensors.

Synergistic action of pesticide mixtures using glutathione-s-transferase- and esterase-inhibiting properties in diamondback moth (Plutella xylostella L.) (Glutathione-S-transferase와 esterase 효소 저해특성을 이용한 농약의 혼합 상승효과)

  • Yu, Yong-Man;Hong, S.S.;Kim, S.;Hur, J.H.
    • The Korean Journal of Pesticide Science
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    • v.7 no.1
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    • pp.38-44
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    • 2003
  • In vitro inhibitory activity of 34 insecticides and 31 fungicides to glutathione-S-transferase and esterases extracted from rats was determined. Of tested pesticides, the pesticides with high activity on both detoxifying enzymes were mixed with pesticides that are known to be detoxified by detoxifying enzymes. Glutathione-S-transferase was inhibited by thiodicarb $(I_{50}:1.87\times10^{-4}M)$, thiocyclam $(7.40\times10^{-4}M)$, dithianon $(7.55\times10^{-5}M)$, and tolylfluanide $(8.66\times10^{-5}M)$, while esterases by dichlorvos $(8.95\times10^{-8}M)$, pirimicarb $(2.74\times10^{-6}M)$, pyrazophos $(3.31\times10^{-5}M)$, and benomyl $(4.96\times10^{-5}M)$. After acephate known to be detoxified by glutathione-S-transferase was mixed with glutathione-S-transferase-inhibiting pesticides and phenthoate known to be detoxified by esterases was mixed with esterases-inhibiting pesticides, insecticidal activities of such mixtures were determined against diamondback moth (PlutelLa xylostella L.). Synergistic action was observed in all pesticide combinations. The highest synergistic action was obtained when phenthoate was combined with dichlorvos, showing that co-toxicity coefficients were 1512 and 1877 after 24 and 48 hours of treatment, respectively. Several other combinations of pesticides, such as phenthoate with benomyl, and acephate with dithianon, also showed synergism, showing that their co-toxicity coefficients were about 1,000 and 500, after 24 hours of treatment, respectively. Our results showed that combinations of pesticides inhibited by detoxifying enzymes and ones detoxified by detoxifying enzymes resulted in increased toxicities of pesticides, suggesting that such combinations could be used to develop pesticide mixtures with more broad spectrum and high effectiveness.

Evaluation of Toxicity of Pesticides against Honeybee (Apis mellitera) and Bumblebee (Bombus terrestris) (꿀벌과 서양뒤영벌에 대한 농약의 독성평가)

  • Ahn, Ki-Su;Oh, Mann-Gyun;Ahn, Hee-Geun;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.12 no.4
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    • pp.382-390
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    • 2008
  • This study was performed to evaluate the acute toxicity and residual toxicity of the 69 kinds of agrochemicals (41 insecticides, 18 fungicides, and 10 acaricides) against honeybee, Apis mellifera and bumblebee, Bombus terrestris. According to the IOBC standard, the toxicity showed below 30% was classified as non-toxic. Among 41 insecticides, five insecticides (acetamiprid, chlorfenapyr, thiacloprid, milbemectin, and buprofezin+amitraz) against the honeybee; eight insecticides (methomyl, thiodicarb, acetamiprid, chlorfenapyr, thiacloprid, abamectin, spino sad, buprofezin+amitraz) against the bumblebee did not show any toxic effect. Therefore, it thought to being safe. Other 18 fungicides and 10 acaricides were safe against the honeybee and bumblebee. In residual toxicity against the honeybee, eight insecticides (dichlorvos, methomyl, imidachlorprid, emamectin benzoate, spinosad, cartap hydrochloride, chlorfenapyr, and endosulfan) among 41 insecticides tested were safe at three days after treatment; however, sixteen insecticides (dimethoate, fenitrothion, fenthion, methidathion, phenthoate, pyraclofos, fenpropathrin, clothianidin, dinotefuran, thiamethoxam, abamectin, acetamiprid+ethofenprox, acetamiprid+indoxacarb, bifenthrin+imidacloprid, ethofenprox+phenthoate, imidacloprid+methiocarb) still remain high toxicity at eleven days after treatment. Against the bumblebee, residual toxicity showed as safe in seven insecticides (dimethoate, methidation, a-cypermethion, ethofenprox, indoxcarb, chlorpyrifos+a-cypennethrin, esfenvalerate+fenitrochion) at three days after treatment; however, eight insecticides (fenitrothion, pyraclofos, clothianidin, fipronil, acetamiprid+ethofenprox, chlorpyrifos+bifenthrin, ethofenprox+phenthoate, imidacloprid+methiocarb) still showed high toxicity at seven days after treatment. From above results, it will be useful information to select insecticides being safe and effective against the honeybee and bumblebee.

Evaluation of Low Toxic and Residual Toxicity of Pesticides Registrated on Sweet Pepper Greenhouse to Orius strigicollis (으뜸애꽃노린재 성충에 대한 착색단고추에 등록된 농약의 저독성 및 잔류독성 평가)

  • Choi, Byeong-Ryeol;Park, Hyung-Man;Kim, Jeong-Hwan;Lee, Si-Woo
    • Korean journal of applied entomology
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    • v.46 no.3
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    • pp.415-423
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    • 2007
  • For the development of integrated pest management system by harmonizing biological and chemical control, some experiments were carried out to select low toxic pesticides and to evaluate residual toxicity to natural enemies. Leaf dipping method and body dipping method were set up for evaluating toxicity to minute pirate bug, Orius strigicollis adult. We had tested 52 kinds of pesticides (33 insecticides, 19 fungicides) commonly used to control greenhouse insects, mites, and disease pests to natural enemies at the recommended concentration. Fourteen insecticides by body dipping method, 12 insecticides by leaf dipping method and 19 fungicides were selected as low toxic pesticides to O. strigicollis adult. After insecticide spraying at recommending dose on the sweet pepper plant, we examined residual effect of insecticides by introducing natural enemies on different days. Safety interval for introduction of O. strigicollis adult was established according to residual toxicity of pesticides. Safety insecticides at one day after treatment were pyraclofos, methomyl, thiodicarb, esfenvalerate bifenthrin, alpha-cypermethrin, etofenprox, fenvalerate, imidacloprid, acetamiprid, abamectin, emamectin benzoate, spinosad, indoxacarb. However, residual toxicity of nee-nicotinoids last up to 21 days to O. strigicollis adults.