• Title, Summary, Keyword: total phenol

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Total Phenol Value in Urine for the Phenol Resin Workers (석탄산 수지 작업자의 뇨중 석탄산총량과 증상)

  • Ahn, Kyu-Dong;Kim, Doo-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.19 no.2
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    • pp.224-232
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    • 1986
  • This study was conducted to evaluate health consequences of phenol resin workers for 6 weeks from February 21, to March 28, 1986. The subject population was 26 in a factory with phenol resin and control group was 30 non-exposed workers who were working in the other factory. The biological parameters chosen for this study were phenol concentration in working room-air, total phenol level in urine and BUN of phenol resin workers. The phenol concentration in working room-air and total phenol in urine were significantly correlated (r=0.791, p<0.01). The frequency of symptom and sign in phenol resin workers were higher than control group. The frequent symptom and sign were coughing, weight loss, poor appetite, headache, dyspnea, eye irritation and tinnitus, in order. Total phenol in urine was not reversed to normal range in spite of interruption of exposure for 4 days. The BUN value in blood and total phenol in urine were not correlated.

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Studies on the Phenol Compounds in Smoke Total Particulate Matters of Flue-cured Tobacco Leaves (황색종 잎담배의 연기응축물중 페놀 화합물에 관한 연구)

  • 복진영;백순옥;김상범;안동명;조수헌
    • Journal of the Korean Society of Tobacco Science
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    • v.23 no.2
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    • pp.162-167
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    • 2001
  • This study was carried out to quantitatively determine phenol compounds in smoke total particulate matter(TPM) to evaluate the qualititive characteristics of flue-cured tobacco varieties(NC82, KFl14 and KFl18). After collecting The TPM by using smoking machine, the concentration of phenol compounds were analyzed by gas chromatography as their trimethylsilyl derivative on a fused capillary column bonded with a 5% PE ME siloxane stationary phase. In all the flue-cured tobacco varieties, the major phenol components were monohydroxy compounds. The order of the highest concentration of total phenol compounds in TPM was NC82, KF114 and KFl18 but the contents of dihydroxy compound in the KFl18 was higher than those of NC82 and KF114.

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Changes of Phenol Compounds according to Storing Years in Soybean

  • Kim, Seung-Hyun;Song, Hong-Keun;Ahn, Joung-Kuk;Kim, Jung-Tae;Hahn, Joon-Sang;Chung, Ill-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.2
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    • pp.82-88
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    • 2004
  • The objective of this study was to determine the role of storing years with the variation of total phenol and individual phenolic compounds in soybean (Glycine max L.) seeds. The total phenol content varied from 0.36 to 0.42% over four years, with the highest value (0.42%) found at storage for two and three years. Among the nine soybean varieties examined, Daweonkong had the highest total average phenol content (0.58%). The total content of 11 phenolics varied from 730.0 to 1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$ over storage for four years, and the highest concentration (1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$) was found at storage for two years. Myeongjunamulkong (1465.4 $\mu\textrm{g}\;\textrm{g}^{-1}$) had the highest mean content among the nine soybean varieties. The total content of 11 phenolic compounds measured in this study occupied from 20.96 to 47.73% of the total phenol contents. The highest total phenol contents were in seeds with black coats (5279.4 $\mu\textrm{g}\;\textrm{g}^{-1}$), while the highest concentration of individual phenolic compounds were in seeds with green coats (1419.5 $\mu\textrm{g}\;\textrm{g}^{-1}$). Our study suggests that it may be feasible to improve soybean varieties with high functional substances such as phenolic compounds.

Effect of Ethanol Solvent Concentration on Antioxidant Activity of Dolwoe (Gynostemma pentaphyllum Makino) Leaves Extracts (돌외 잎 추출물의 항산화 활성에 미치는 에탄올 용매 농도의 영향)

  • Kim, Kyeoung Cheol;Kim, Ju-Sung
    • Journal of Advanced Engineering and Technology
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    • v.11 no.3
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    • pp.197-203
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    • 2018
  • The purpose of this study was to the extraction availability of Gynostemma pentaphyllum leaves on different ethanol concentration extraction solvent were compared and analyzed. We prepared extracts of different solvents. Total phenol and flavonoid contents of each extract were measured. These extracts were evaluated for antioxidant activity by measuring trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAC), oxygen radical absorption capacity (ORAC), DPPH, nitric oxide, superoxide and hydroxyl radical scavenging activity. The total phenol contents of extracts was 44.16~66.34 mg QAE/g, and the total phenol content in the extracts was significantly increased when ethanol concentration of 60% or more was used. Total flavonoid content was 6.98~16.89 mg QE/g and 60, 80% ethanol extracts were significantly higher. In the antioxidant experiments, 60% ethanol extracts showed high activity in nitric oxide, superoxide radical scavenging activity, TEAC and ORAC which showed correlation with total phenol and flavonoid contents. The optimal ethanol concentration used for the extraction of antioxidant-related components from Gynostemma pentaphyllum leaves is considered to be about 60%, antioxidant activity was thought to be related to total phenol and flavonoid contents.

Comparision of SOD Activity and Phenolic Compound Contents in Various Korean Medicinal Plants (한국산 약용식물로부터의 SOD활성 및 Phenolic Compounds 함량 비교)

  • Lim, Jung-Dae;Yu, Chang-Yeon;Kim, Myong-Jo;Yun, Song-Joong;Lee, Sun-Ju;Kim, Na-Young;Chung, Ill-Min
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.3
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    • pp.191-202
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    • 2004
  • 82 species of Korean medicinal plants were measured to their SOD (superoxide dismutase) activity, total phenol and individual phenol compounds. The SOD activity of medicinal plants was varied from 3.67% to 48.3%, and especially, 4 species including Polygala tenuifolia Willd. showed activity over 40% higher than other species. The 16 individual phenol compounds concentrations have a severe variation in medicinal plants. Codonopsis lanceolata is the highest concentration $(12,176\;{\mu}g/g)$, and 10 species including Glycyrrhiza uralensis Fisch were showed to concentrations more than $1,000\;{\mu}g/g$. And also, salicylic acid was generally found in many plants, but trans-cinnamic acid, naringenin and kampferol was little found in plants. The total phenol by Folin-Dennis methods showed a respectively variation $(3{\sim}249.731\;{\mu}g/g)$ in 82 medicinal plants, and 7 species including Astragalus membranaceus showed low total phenol concentrations less than $10\;{\mu}g/g$. In conclusion, we expect that selecting medicinal plants with high biological activity might develop to chemicals related to antioxidants, as well as these become most popular substance at many industrial materials, and suggest to more researchs in future.

Comparison of Total Phenol, Flavonoid Contents, and Antioxidant Activities of Orostachys japonicus A. Berger Extracts (와송(Orostachys japonicus A. Berger) 추출물의 총 페놀, 플라보노이드 함량 및 항산화 활성 비교)

  • Jin, Dong-Hyeok;Kim, Han-Soo;Seong, Jong-Hwan;Chung, Hun-Sik
    • Journal of Environmental Science International
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    • v.25 no.5
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    • pp.695-703
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    • 2016
  • The purpose of this study was to measure the bioactivity and antioxidant activity of Orostachys japonicus A. Berger. Orostachys japonicus A. Berger have been known to contain functional materials such as kaempferol, hydroquinone, methyl gallate, quercetin, gallic acid etc. To identify the main functional materials of Orostachys japonicus A. Berger, the contents of flavonoid and phenol were measured. We extracted Orostachys japonicus A. Berger powder from four solvents such as chloroform:methanol (CM, 2:1, v/v), distilled water (DW), 70% methanol, 70% ethanol. After that, this study determined tannin, total phenol, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power and reducing power of Orostachys japonicus A. Berger extracts and as results of comparing each extract. respectively. From the above results shows that antioxidant activity and bioactivity of Orostachys japonicus A. Berger extracts was higher in the order of 70% ethanol, 70% methanol, DW and CM (p<0.05). The results showed that antioxidant activity of Orostachys japonicus A. Berger extracts supposed to affect by the total phenol and flavonoid contents.

Changes of Biological Activities of Rhizome and Leaves of Polygonatum sibiricum Redoute according to Steaming Time and Temperature (층층갈고리둥굴레 (Polygonatum sibiricum Redoute) 뿌리줄기와 잎의 증숙 시간과 증숙 온도에 따른 생리활성 변이 연구)

  • Oh, Yeong Seon;Choi, Jae Hoo;Kim, Chul Joong;Seong, Eun Soo;Kim, Myong Jo;Yu, Chang Yeon;Lee, Jae Geun
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.5
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    • pp.331-338
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    • 2020
  • Background: The aim of this study was to analyze the total phenol and total flavonoid contents and antioxidant activity of steam-treated leaves and rhizomes of Polygonatum sibiricum Redoute. In addition, we aimed to confirm their potential use as cosmetic materials by investigating their anti-aging and skin-whitening activity. Methods and Results: The leaves and rhizomes of P. sibiricum were treated with steam at different temperatures for different durations, and the antioxidant activity (DPPH and ABTS radical scavenging activity) and total phenol and total flavonoid contents of each sample were tested. The steam temperature and treatment duration siginificantly affected the antioxidant activity and, total phenol and total flavonoid content of the leaves and rhizome of P. sibiricum. Treating the P. sibiricum samples with steam at 120℃ for 12 h, yielded higher total phenol and total flavonoid contents. Comparatively, the samples treated with steam at 120℃ for 12 to 24 h showed significantly higher antioxidant activity. Further, the steamed samples of P. sibiricum demonstrated collagenase and tyrosinase inhibition activity, which indicated their anti-aging and skin-whitening properties. The samples steamed at 120℃ for 12 h, exhibited higher collagenase and tyrosinase inhibition activity. Conclusions: Leaves and rhizomes of P. sibiricum steamed at 120℃ for 12 h, showed highest antioxidant activity and, total phenol and total flavonoid contents than all other samples. Our results indicate the potential of using P. sibiricum as a cosmetic material by confirming its excellent anti-aging and whitening activity.

Effects of Various Extracts from Turmeric (Curcuma longa L.) on Antioxidant Activity

  • Kim, Gyeong-Hwuii;Duan, Yishan;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.3
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    • pp.521-528
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    • 2016
  • The antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from turmeric (Curcuma longa L.) was investigated in this study. The antioxidant potential of various extracts of turmeric was evaluated by using different antioxidant tests, namely DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ferric reducing antioxidant power and ${\beta}$-carotene bleaching assays as well as total phenol content and total flavonoid content. All extracts exhibited abundant total phenol content, total flavonoid content and strong antioxidant activity. Particularly, higher activity was exhibited by CM extract with $46.32{\pm}0.35mgCAE/g$ and $15.58{\pm}0.39 mgQE/g$ of total phenol content and total flavonoid content. Generally, antioxidant activity of various extracts from turmeric increased in the following order: 70% methanol extract, 70% ethanol extract and CM extract, respectively. Results suggested that turmeric could be widely used as a source of antioxidant and a kind of functional material in food.

Screening of the total phenol content and analysis of phenolic compound in rice (Oryza saiva L.) genetic resources

  • Lee, Ji-Hee;An, Min-Jeong;Kim, Seung-Hyun;Chung, Ill-Min
    • Proceedings of the Korean Society of Crop Science Conference
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    • pp.290-290
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    • 2017
  • Rice (Oryza sativa L.) is one of the most consumed staple food crop which is energy source as carbohydrate and also is considered as the important antioxidant sources including various phenolic compounds. According to the increasing demand of healthy life, the concern to antioxidant also is increasing because of its health-promoting effect. Phenolic compounds are one of the plant secondary metabolites class, which shows various benefits to preventing or treating chronic diseases. In this study, we have measured the total phenol content from total 647 rice samples using the Floin-Ciocalteau method, and then were selected 30 rice genetic resources classified with high, middle, and low group on the basis of total phenol content. The average of the total phenol content of each group was high-group ($6892.9{\pm}488.5{\mu}g\;GAE/g$) > middle-group ($1428.1{\pm}76.0{\mu}g\;GAE/g$) > low-group ($97.6{\pm}11.4{\mu}g\;GAE/g$). The selected rice samples were analyzed with LC-MS/MS to find the composition and concentration of individual phenolic in rice grain. High-group and middle-group contained large amounts of protocatechuic acid and (+)-catechin whereas low-group showed limited amount. Among high-group samples, rice samples with black pericarp color (IT 174089, IT 220079, and IT 259958) had high content of peonidin-3-O-glucoside. Further, these black rice samples were special since polydatin, rarely found stilbenoid in rice grain, was detected. Overall, both the sum of phenolic acid and the sum of flavonoid were high-group > middle-group > low-group. Also, each group exhibited different phenolic compositions; high-group consisted of flavonoid more than phenolic acid, middle-group and low-group was comprised of phenolic acid rather than flavonoid, and non-pigmented rice was composed by fully phenolic acid. The total phenol content had positive relationships with the sum of phenolic compound (r = 0.64), the sum of flavonoid (r = 0.74) at the significance level of p < 0.0001. In addition, protocatechuic acid and quercetin showed positive correlation with above phenolic composition parameters; in order, r = 0.98, 0.65 for protocatechuic acid and r = 0.73, 0.78 for quercetin (p < 0.0001). In conclusion, the total phenol content assay showed the possibility of utilization as a phenolic composition indicator in rice grain. Also, this result was suggested study pigment on other material.

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Antioxidant Activity and In Vitro Protein Digestibility of Bakery Products prepared from Korean Wheat with Functional Ingredients (기능성 소재를 첨가한 우리밀 제빵 제품의 항산화 활성과 단백질 소화도)

  • Han, Inhwa;Park, Byung Geon
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.6
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    • pp.853-861
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    • 2014
  • Cookies, muffins and baguettes were formulated with Korean wheat flour and compared with those made from imported wheat flour in terms of total phenol content, antioxidant activity, and in vitro protein digestibility (IVPD). Chunnyencho and Honghawsi were added to improve quality of the bakery products. Addition of Chunnyencho did not show any significant improvement, whereas Honghawsi increased total phenol content of cookies. Total phenol content in 80% ethanol extract of muffins prepared with Korean wheat flour was 1.5 times higher than those made from imported wheat flour, whereas total phenol content was 1.2 times higher in the 80% methanol extract of baguettes prepared with imported wheat flour compared to those made with Korean wheat flour. Overall, total phenol content of Korean wheat flour was higher than that of imported wheat flour because total phenol content was higher in 80% ethanol extracts than in 80% methanol extracts. Antioxidant activity was not significantly different between ethyl acetate extracts of bakery products from imported wheat and Korean wheat, whereas antioxidant activity of 80% methanol extract of muffins with Korean wheat was 2.4 times higher than that from imported wheat. IVPD was not significantly different in any bakery products made from imported wheat or Korean wheat but was higher in baguettes than cookies or muffins. Conclusively, bakery products made from Korean wheat had higher total phenol content and antioxidant activity than imported wheat.