• Title, Summary, Keyword: total phenolic contents

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The determination of total phenolic and flavonoid contents in Lentinus edodes and their Glutathione S-Transferases effects

  • Hwang, Hyun-Ik;Lee, In-Son;Moon, Hae-Yeon
    • 한국생물공학회:학술대회논문집
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    • pp.667-671
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    • 2003
  • The total phenolic and flavonoid contents of Lentinus edodes extracts were determinated by spectrophotometrical .method, and antihepatotoxic activity was detected on glutathione S-Transferases(GST). The total phenolic contents was highest water extract than solvent(ethanol, methanol) extracts, but flavonoid content was appear on opposite. GST activity was the highest appears in water extract. This fact verified of anticancer effect indirectly of Lentinus edodes.

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Increasement of Eleutherosides and Antioxidant Activity in Eleutherococcus senticosus Adventitious Root by Jasmonic acid

  • Ahn, Jin Kwon;Park, Youngki;Lee, Wi Young;Park, So-Young
    • Journal of Korean Society of Forest Science
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    • v.96 no.5
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    • pp.539-542
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    • 2007
  • This study was carried out to investigate the impacts of jasmonic acid (JA) on adventitious root culture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid (JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, total phenolic contents and antioxidant activity. Among various concentrations of JA, 1.0 mg/L JA increased the total phenolic contents in E. senticosus adventitious root to $39.81{\mu}g/g$, about 2.6 times higher than that of the control. Consequently, high accumulation of total phenolic contents led to increase the antioxidant activity to 82.41%. The antioxidant activity of control was 37.89% at $2500{\mu}g/mL$. A linear correlation ($R^2$ = 0.9937, 0.9648 and 0.9883) was also shown between antioxidant activity (at 1250, 1875, and $2500{\mu}g/mL$) and total phenolic contents of adventitious root of E. senticosus.

Changes in Total Polyphenol Contents and DPPH Radical Scavenging Activity of Agrimonia pilosa According to Harvest Time and Various Part (수확시기별, 부위별 선학초의 폴리페놀함량 및 DPPH 라디컬 소거능의 변화)

  • Jang, Sang-Hun;Yu, Eun-Ae;Han, Ki-Soo;Shin, Sung-Chul;Kim, Hee-Kyu;Lee, Sang-Gyeong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.6
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    • pp.397-401
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    • 2008
  • Changes in the contents of total phenolic compounds in as Agrimonia pilosa well as their antioxidant capacity according to the havest time and positions were examined. The contents of the total phenolic compounds were determined by extraction with MeOH. Among havest times harvestry in July showed highest contents of the total phenolic compounds and harvestry in May showed lowest contents of the phenolic compounds. Among the 4 positions (root, branch, leaf, flower) of Agrimonia pilosa the root contained highest contents of the phenolic compounds. The antioxidant capacities of Agrimonia pilosa were increased roughly with increasing level of contents of phenolic compounds according to positions.

Characteristics and Antioxidant Effect of Garlic in the Fermentation of Cheonggukjang by Bacillus amyloliquefaciens MJ1-4

  • Kim, Jeong Hwan;Hwang, Chung Eun;Lee, Chang Kwon;Lee, Jin Hwan;Kim, Gyoung Min;Jeong, Seong Hoon;Shin, Jeong Hee;Kim, Jong Sang;Cho, Kye Man
    • Journal of Microbiology and Biotechnology
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    • v.24 no.7
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    • pp.959-968
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    • 2014
  • The changes in the ${\beta}$-glucosidase activity, total phenolic contents, isoflavone contents, and antioxidant activities during the fermentation of cheonggukjang by Bacillus amyloliquefaciens MJ1-4 with and without garlic were investigated. The levels of total phenolic and isoflavone-malonylglycoside, -acetylglycoside, and -aglycone contents increased, whereas the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and ferric reducing/antioxidant power (FRAP) assay results increased, but isoflavone-glycoside levels decreased during cheonggukjang fermentation. The levels of total phenolic and total isoflavone contents and the antioxidant activities were higher in cheonggukjang fermented without garlic (CFWOG) than in cheonggukjang fermented with garlic (CFWG) after 24 h of fermentation, but they were lower in CFWOG than in CFWG after 72 h of fermentation. In particular, the highest levels of total phenolic, daidzein, glycitein, and genistein were present at concentrations of 15.18 mg/g, $264.4{\mu}g/g$, $16.4{\mu}g/g$, and $31.1{\mu}g/g$ after 72 h of fermentation in CFWG, showing 82.89% in DPPH radical scavenging activity, 106.32% in ABTS radical scavenging activity, and 1.47 ($OD_{593nm}$) in FRAP assay, respectively. From these results, we suggest that the high antioxidant activity of CFWG might be related to the markedly higher levels of total phenolic contents, isoflavone-malonylglycosides, -acetylglycosides, and -aglycones achieved during fermentation.

Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times (수삼의 증숙 횟수에 따른 페놀산 함량 변화와 라디칼 소거활성)

  • Kim, Young-Chan;Hong, Hee-Do;Rho, Jeong-Hae;Cho, Chang-Won;Rhee, Young-Kyung;Yim, Joo-Hyuk
    • Journal of Ginseng Research
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    • v.31 no.4
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    • pp.230-236
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    • 2007
  • This study was conducted to investigate the contents of the total phenolic compounds, and DPPH, ABTS radical scavenging activities of phenolic acid fractions of ginseng according to steaming times. Also the individual phenolic acid compositions and contents were analyzed by GC. The contents of the total phenolic compounds proportionally increased from 0.530 to 2.893% according to steaming times. Phenolic acid fractions were separated according to bound types, and the insoluble bound form fraction showed the highest contents followed by ester form fraction and free form fraction. The total contents of these three fractions (1.031-1.416%) were not significantly influenced by steaming times. Salicylic, cinamic, p-hydroxybenzoic, gentisic, vanillic, syringic, caffeic, ferolic acid were found in each fraction, and gentisic and ferolic acid were the major phenolic acid. Each phenolic acid fraction showed over 50% of DPPH and ABTS radical scavenging activities. There were no differences between the phenolic acid fractions according to binding types. Free radical scavenging activities were affected by a number of steaming times and augmented as steaming times increased.

Total Phenolic Contents and Biological Activities of Korean Seaweed Extracts

  • Kim, So-Jung;Woo, Seon-Ock;Yun, Hee-Young;Yum, Seung-Shic;Choi, Eun-Seok;Do, Jeong-Ryong;Jo, Jin-Ho;Kim, Dong-Giun;Lee, Suk-Chan;Lee, Taek-Kyun
    • Food Science and Biotechnology
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    • v.14 no.6
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    • pp.798-802
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    • 2005
  • Crude extracts of thirty seaweeds collected in Korea were obtained using 50% ethanol, and total phenolic contents and antioxidant activities were compared. Two brown algae, Ecklonia cava (E. cava) and Sargassum siliquastrum (S. siliquastrum), showing high antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and superoxide anion radical scavenging activity were further investigated for their inhibitory effects on tyrosinase activity. The E. cava extract had the highest total phenolic content among the seaweeds extracts. Total phenolic contents were strongly correlated with antioxidant activity in the thirty seaweed extracts ($R^2\;=\;0.9169$). The E. cava and S. siliquastrum extracts exhibited higher inhibition to tyrosinase activity than butylated hydroxytoluene (BHT) and epigallocatechin gallate (EGCG).

Changes in Physicochemical Properties and Antioxidant Activities of Brown Rice (Oryza sativa L.) throughout Germination

  • Oh, Sea-Kwan;Lee, Jeong-Huei;Hwang, Hung-Goo;Lee, Dong-Hyeon;Kim, Yeon-Gyu;Lee, Jin-Hwan
    • Preventive Nutrition and Food Science
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    • v.15 no.3
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    • pp.221-228
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    • 2010
  • The objective of this research was to investigate the changes in the contents of physicochemical properties including $\gamma$-aminobutyric acid (GABA), total dietary fiber (TDF), amylose, protein, and fat content in brown rice through germination for 2 different years. Total phenolic contents and antioxidant activities of DPPH and ABTS radical scavenging capacities were also determined in different solvent extracts. For the physicochemical properties, GABA, TDF, protein, and fat content increased, whereas amylose levels decreased. Specially, GABA and TDF levels showed the greatest variations among cultivars and harvest years. Total phenolic content and antioxidant activity significantly increased. The average total phenolic content at a concentration of 0.5 mg/mL in different extract solvents occurred in this order: methanol>ethylacetate>chloroform>hexane extracts. Additionally, 'Keunnun' exhibited the highest GABA levels, highest total phenolic content, and highest antioxidant activity after germination, with increases of approximately 3.7, 2.0, and 1.9 times, respectively, compared to levels before germination. These results suggest that, because of its high physicochemical contents and strong radical scavenging activities, germinated brown rice can be used as beneficial supplement.

Antioxidant Activity and Total Phenolic Content of Callistemon citrinus Extracts

  • Park, Young-Ki;Lee, Wi-Young;Park, So-Young;Ahn, Jin-Kwon;Han, Mu-Seok
    • Food Science and Biotechnology
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    • v.14 no.2
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    • pp.212-215
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    • 2005
  • Ethanol crude extracts of wood, bark, leaf, and fruit of Callistenwn citrinus were compared for their antioxidant activities based on DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging activity and reducing power. Bark extract showed the most potent radical-scavenging activity and reducing power, showing 94.1 and 0.64% at 25 and $100\;{\mu}g/mL$, respectively. Total phenolic content of the bark extracts (275.0 mg GAE/g) was higher than those of others. Further fractionation of the bark extract using hexane, $CH_2Cl_2$, and EtOAc showed EtOAc fraction had the highest antioxidant activity ($IC_{50}\;6.7\;{\mu}g/mL$) and reducing power (0.82 at $100\;{\mu}g/mL$), with total phenolic content of 611.1 mg GAE/g. Total phenolic contents correlated with antioxidant activity ($R^2\;=\;0.7061$) and reducing power ($R^2\;=\;0.7399$).

Antioxidant Activity of Methanol Extracts from Cudrania tricuspidata Bureau according to Harvesting Parts and Time (이용부위 및 채취시기에 따른 꾸지뽕나무 메탄올 추출물의 항산화성)

  • Choi, So-Ra;You, Dong-Hyun;Kim, Jong-Yeob;Park, Chun-Bong;Kim, Dae-Hyang;Ryu, Jeong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.2
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    • pp.115-120
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    • 2009
  • To obtain the information on antioxidant activity by harvesting parts and time in Cudrania tricuspidata Bureau, total phenolic contents, flavonoid contents, superoxide dismutase (SOD) like activity and 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity of methanol extracts from Cudrania tricuspidata were investigated. Total phenolic contents was the highest in leaf [$30.2{\sim}38.8\;mg/g$ dry weight (DW)] and followed by root bark, stem bark, fruit, root except bark and stem except bark. Among harvesting time, total phenolic contents of leaf was the highest as $38.1{\sim}38.8\;mg/g$ DW in May and June, and then decreased to October. Root bark harvested in April and May contained $26.6{\sim}27.8\;mg/g$ DW total phenolic compounds and total phenolic content fell to a very low values at 1.8 mg/g DW in February. Flavonoid contents was very high in leaf and root bark. Especially, root bark harvested in April had the highest of flavonoid contents as 23.2 mg/g DW. As a result of simple linear regression analysis of total phenolic contents on flavonoid contents according to harvesting parts, it showed a high correlation (p < 0.001) with $0.57{\sim}0.97\;R^2$ (coefficient of determination). As $RC_{50}$ value, that was, the concentration of sample required for 50% reduction of DPPH absorbance, was $13.7{\sim}20.5\;{\mu}g/mL$ in leaf so DPPH radical scavenging activity was very high. But SOD like activity of $1,000\;{\mu}g$ extract/mL MeOH was a low value of $0.3{\sim}9.2%$ in all samples.

Effect of Drying Method on Antioxidant Activity of Jiwhang (Rehmannia glutinosa)

  • Rhim, Jong-Whan;Xi, Yang;Jeong, Won-Chul;Ham, Kyung-Sik;Chung, Ha-Sook;Kim, Eun-Sil
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1464-1469
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    • 2009
  • Jiwhang (Rehmannia glutinosa), one of the most widely used medicinal herbs, was dried with various methods such as sun drying, hot air drying, vacuum drying, and freeze drying methods, and their effects on the antioxidant capacity in relation with the content of total phenolic compounds were studied with a steamed-and-dried rehmannia (sookjiwhang) for comparison. Generally, total phenolic contents decreased significantly by all of the drying treatments except the steamed-and-dried rehmannia, in which total phenolic contents increased 2.4 fold compared with fresh rehmannia. Content of verbascoside, a functional phenolic compound, was the highest in the freeze-dried rehmannia ($177.97{\pm}0.02\;{\mu}g/g$ d.m.) followed by vacuum-dried ($105.55{\pm}0.07\;{\mu}g/g$ d.m.), hot air-dried ($23.01{\pm}0.02\;{\mu}g/g$ d.m.), and sun-dried ($4.89{\pm}0.13\;{\mu}g/g$ d.m.) ones comparable to the fresh rehmannia ($80.15{\pm}1.26\;{\mu}g/g$ d.m.). Antioxidant capacity determined by both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) methods agreed with the result of total phenolic contents, that is, the antioxidant capacity was the highest in the steamed-and-dried rehmannia followed by fresh rehmannia, vacuum-dried, hot air-dried, sun-dried, and freeze-dried ones. Conclusively, the total phenolic contents and antioxidant capacity of rehmannia were greatly affected by the drying methods used.