• Title/Summary/Keyword: water deficit

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Antioxidant capacity in seedling of colored-grain wheat under water deficit condition

  • Kim, Dae Yeon;Hong, Min Jeong;Jung, Woo Joo;Seo, Yong Weon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.140-140
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    • 2017
  • Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

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Regulated Deficit Irrigation and Its Several Problems in Practical Use

  • Huanjie Cai;Shaozhong Kang
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.42
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    • pp.31-37
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    • 2000
  • Regulated Deficit Irrigation (RDI) is one of the most important measures for the water-saving and high yield of crops. RDI is based on the crop and water relations. The theories of RDI were analyzed using the experiment data in Shaanxi and Gansu Province. There are several problems of RDI in practical use, which include; the uncertainty of crop-water relations, the proper growth stages and water deficit degree of RDI applied, and the requirements of RDI to irrigation system and irrigation techniques.

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Protective Effect of Artificially Enhanced Level of L-Ascorbic Acid against Water Deficit-Induced Oxidative Stress in Rice Seedlings

  • Boo, Yong Chool;Cho, Moonjae;Jung, Jin
    • Journal of Applied Biological Chemistry
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    • v.42 no.2
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    • pp.66-70
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    • 1999
  • Effects of the enhanced level of L-ascorbic acid (AA) on the water deficit-induced oxidative damage were studied in rice (Oryza sativa L.) seedlings. The seedlings sprayed with 20 to 80 mM L-galactono-${\gamma}$-lactone (GL), a putative precursor of AA, showed 2 to 5-fold higher levels of AA compared with controls. Pretreatment of the seedlings with GL prior to water stress imposition caused virtually no effect on dehydration of tissues during water deficit but substantially mitigated oxidative injury, as accessed by 2-thiobarbituric acid-reactive substances, ${\alpha}$-tocopherol, chlorophylls and ${\beta}$-carotene. Proline accumulation during water stress was also significantly lowered in the treated seedlings. In a complementary experiment, AA retarded photodegradation of ${\alpha}$-tocopherol in isolated thylakoids far more efficiently than glutathione. GL in itself did not show any noticeable reactivity toward ${\alpha}$-tocopheroxyl radical. The results demonstrate the antioxidative function of AA in rice seedlings encountering water-limited environments, suggesting a critical role of AA as a defense against oxidative stress in plants.

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Effects of Endomycorrhizal Glomus Inoculation on Drought Resistance and Physiological Changes of Lespedeza cyrtobotrya Seedlings Exposed to Water Stress (Glomus 내생균근균(內生菌根菌) 접종(接種)이 수분(水分) 스트레스에 노출(露出)된 참싸리의 건조저항성(乾燥抵抗性) 및 생리적(生理的) 변화(變化)에 미치는 영향(影響))

  • Kim, Hyo-Jin;Lee, Kyung-Joon;Han, Sim-Hee
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.2
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    • pp.53-62
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    • 2002
  • The objectives of this study were to understand the tolerance mechanism of woody plants to water stress and tolerance changes in relation to mycorrhizal formation. Lespedeza cyrtobotrya Miq. commonly used for erosion control in slopes were raised from seeds and transplanted to 120 plastic pots. Sixty pots received the top soil of a Fraxinus americana forest, while remaining 60 pots received the autoclaved top soil. The forest soil contained 1,200 spores per 100g of arbuscular endomycorrhizal fungus, mostly Glomus sp. The plants were raised outside with regular supply of water and mineral nutrients. Two kinds of water deficit treatment and a control were started at the middle of July : cyclic water deficit treatment with 3 cycles of sequential water stress at the point of xylem water potential of about -0.6, -0.6, and -1.7 MPa and recovery, and non-cyclic water deficit treatment with single water stress at about -1.5 MPa. The non-stressed plants received plenty of water throughout the period. In late August the plants were harvested for measurements of dry weight, N, P, carbohydrate contents, net photosynthesis and superoxide dismutase(SOD) activities. Both cyclic and non-cyclic water deficit treatments reduced dry weight by 60% and 40%, respectively, and reduced nitrogen absorption, while increased SOD activities. Water-stressed plants also showed increased carbohydrate contents in the leaves and lowered stomatal conductance. Mycorrhizal inoculation resulted in an average of 40% infection of roots and 2-3 times increase in P absorption in water-stressed as well as non-stressed plants. Mycorrhizal formation also increased shoot-root ratio. The results that SOD activities of water-stressed plants with mycorrhizal infection were significantly lower than those of non-mycorrhizal plants suggest the possibility of improvement of water-stressed condition by mycorrhizal formation. It was concluded that endomycorrhizal formation increased tolerance of Lespedeza cyrtobotrya seedlings to water stress.

Effects of salicylic acid on growth and proline of cucumber seedlings

  • Kim, Tae-Yun;Lee, Gui-Soon;Jin, Sun-Young;Hong, Jung-Hee
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2002.05b
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    • pp.465-467
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    • 2002
  • The effects of salicylic acid (SA) on growth and proline were investigated in cucumber seedlings. Exogenous application of SA(100 uM - 1mM) led to a noticeable decrease in root and shoot growth, and dry weights of seedlings. Anatomical observation on leaf anatomy of cucumber revealed that the thickness of all leaf tissue components decreased in SA-treated plants. The effect was most promounced on the width of the adaxial epidermis. In the separate and simultaneous effects of SA and water deficit induced by PEG on growth and proline accumulation, the water deficit treatments had greater effects on growth traits and proline content than SA. Combinations of SA and PEG decreased dry matter and root length, and resulted in higher proline in both shoots and roots than SA stress alone. Shoots had higher proline than roots.

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Regulated Deficit Irrigation and Its Several Problemsin Practical Use (부족조절관개와 실용상의 문제점)

  • Cai, Huanjie;Hang, Shazhong
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1999.10c
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    • pp.30-40
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    • 1999
  • Regulated Deficit Irrigation (RDI) is one of the most important measures for the watcr-saving and high yield of crops. RDI is based on the crop and water relations. The theories of RDI were analyzed using the experiment data in Sha.anxi. and Gansu Province. There are several problems of RDI in practical use, which include: the uncertainty of cropwwater relations, the proper growth stages and water deficit d$\xi$gree ofRDI applied, and the requirements ofRDI to irrigation system and irrigation tecbniques.

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Assessment of Remote Sensing-based Hydrological Drought Indices (인공위성영상 기반의 수문기상변수를 활용한 수문학적 가뭄지수 개발 및 평가)

  • Sur, Chanyang;Park, Seo-Yeon;Kim, Tae-Woong;Lee, Joo-Heon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.22-22
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    • 2018
  • 본 연구에서는 수문학적 가뭄을 분석하기 위해 두가지 지수를 개발하여 소개하고자 한다. 첫번째는, 물수지식을 기반으로 산정된 Water Budget-based Drought Index(WBDI)로 강우와 증발산의 차이를 이용하여 산정한다. 두 번째는 에너지 수지식을 기반으로 산정된 Energy-based Water Deficit Index(EWDI)로 에너지 수지 기반의 증발산, 태양복사에너지와 토양수분 등을 이용하여 산정한다. 두가지 지수 모두 인공위성 영상 자료를 활용하였다. WBDI 산정을 위한 강수량 자료는 Tropical Rainfall Measuring Mission(TRMM)과 Global Precipitation Mission(GPM)를 활용하였으며, 증발산 자료는 Moderate Resolution Imaging Spectroradiometer (MODIS) 자료를 활용하였다. EWDI 산정에 필요한 입력자료는 모두 MODIS 자료를 활용하였다. 산정된 두 가뭄지수의 수문학적 가뭄 분석을 위해 자연유출지점인 6개 지점을 선정하여 유출량 자료와 비교하였다. 유출량 자료를 활용하여 Error matrix 기법을 적용하여 두 수문학적 가뭄지수의 우리나라에서의 적용성을 파악하였다.

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