• Title, Summary, Keyword: xanthine oxidase

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Analysis of Relationship Between Spermatozoa Ability and Reactive Oxygen Species in Porcine: I. Sperm Preincubation by Xanthine and Xanthine Oxidase (돼지정자의 수정능력과 Reactive Oxygen Species의 관계분석 I. Xanthine과 Xanthine Oxidase에 의한 정자의 전배양)

  • Park, C.K.;Cheong, H.T.;Kim, J.H.;Lee, S.C.;Yang, B.K.;Kim, C.I.
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.3
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    • pp.315-322
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    • 1998
  • The objective of this study was to test the effect of catalase on penetration in vitro by spermatozoa preincubated with xanthine and/or xanthine oxidase. The penetration rates were, significantly (p<0.05) higher in spermatozoa preincubated without (66 and 38%) than with (40 and 15%) catalase for 0 and 30 min. When spermatozoa were preincubated and inseminated in medium with xanthine, the penetration rates were significantly higher (p<0.05) in medium with (68, 70 and 49% for 0, 30 and 60 min) than without (33, 41 and 19% for 0, 30 and 60 min) catalase. However, in oocytes were' inseminated with spermatozoa pre incubated with or without catalase in the presence of xanthine oxidase, no decrease in penetrations rates were observed for up to 60 min of preincubation. In another experiment, the penetration rates were significantly (p<0.00l) higher in medium with (75, 55 and 52%) than without (14, 4 and 8%) catalase when oocytes were inseminated with spermatozoa preincubated for 0, 30 and 60 min in the presence of xanthine plus xanthine oxidase. On the other hand, The rate of polyspermy in oocytes penetrated in medium without catalase in the presence of xanthine or xanthine plus xanthine oxidase decreased with time of spermatozoa preincubation. However, no differences were observed in polyspermy rates in the medium with xanthine oxidase alone despite presence of catalase. These results indicate the advantages of spermatozoa pre incubated with xanthine plus xanthine oxidase in the presence of catalase to increase penetration potential and with suppressed polyspermy in porcine.

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Inhibitory Effect of Condensed Tannins Isolated from Korean Green Tea against Xanthine Oxidase (한국산 녹차로부터 분리한 축합형 탄닌의 Xanthine Oxidase 저해효과)

  • Cho, Young-Je;Chun, Sung-Sook;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.418-422
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    • 1993
  • For the purpose of utilizing tannins in the functional foods and crude drugs the xanthine oxidase inhibition of tannins isolated from Korean green tea was determined. Acetone extract from Korean green tea showed inhibitory effect against the xanthine oxidase. The galloyl tannins showed higher inhibitory activity against xanthine oxidase than the nongalloyl tannins. In terms of stereo isomers, (-)-epicatechins had higher inhibitory activity than the (+)-catechins. The synergistic activity was also observed. Tannins isolated from Korean green tea appeared to be incompetitive inhibitor against the xanthine oxidase.

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Free Radical Scavenging Activities and Inhibitory Effects on Xanthine Oxidase by Ethanol Extract from Capsella bursa-pastoris (냉이(Capsella bursa-pastoris)에탄을 추출물의 유리라디칼 소거 및 Xathine Oxidase 저해활성)

  • Hong, Jung-Il;Ra, Kyung-Soo;Sung, Ha-Chin;Yang, Han-Chul;Kweon, Mee-Hyang
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.590-595
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    • 1995
  • To examine the characteristics of antioxidative compounds from Capsella bursa-pastoris, ethanol extracts were separated into five organic solvent fractions; hexane(Fr.H), diethyl ether (Fr.E), ethyl acetate(Fr.EA), butanol (Fr.B), and water(Fr.D) fractions. Fr.B showed the greatest electron donating ability and inhibitory effect on lipid peroxidation. Whereas Fr.E had the most excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. The inhibitory effect of each fraction on xanthine oxidase was also measured. Fr.E had the strongest inhibitory effect on xanthine oxidase and $IC_{50}$ was $5.65\;{\mu}g$. The results indicate that the superoxide radical scavenging activity of Fr.E is caused by the inhibitory effect on radical generating system of xanthine oxidase. Also the order of inhibitory effect on xanthine oxidase was Fr.B

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Effect of Methanethiol on the Xanthine Oxidase Activity of Liver and Serum in Rats (흰쥐에 Methanethiol 투여가 간 및 혈청 Xanthine Oxidase 활성에 미치는 영향)

  • 윤종국;정소웅;이상일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.3
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    • pp.209-213
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    • 1991
  • To evaluate the effect of methanethiol on the activity of xanthine oxidase in both liver and serum, the rats intraperitoneally received methoanethiol. Injection of methoanethiol in rats showed the more decreased xanthine oxidase activity of boty liver and serum than that of the control group. Concomitantly, the xanthine oxidase activity in livers preincubated with methanethiol was decreased in vitro. The xanthine oxidase in livers preincubated with the methoanethiol also showed more increased Km and similar Vmax value than those of the control. These results suggest that the methanethiol may induce a change in substrate binding affinity of xanthine oxidase.

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Effect of Lead Ion on The Hepatic Xanthine Oxidase Activity in Vitro (납이온이 잔틴 옥시다제 활성에 미치는 영향)

  • Huh, Keun;Shin, Uk-Seob;Lee, Sang-Hoon;Ann, Won-Hyo
    • YAKHAK HOEJI
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    • v.39 no.5
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    • pp.521-527
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    • 1995
  • This study was done to determine the effect of lead acetate on the activities of the hepatic cytosofic xanthine oxidase and aldehyde oxidase which were well known as oxygen free radical generating enzyme in vitro. Lead ion accelerated the formation of lipid peroxide and the increment of xanthine oxidase(type O) activity and the type conversion ratio from xanthine dehydrogenase to xanthine oxidase dose-dependently. But xanthine dehydrogenase(type D) activity was decreased. Aldehyde oxidase activity was not changed by lead ion. These data suggested that lead-induced cellular to)dcity may be concerned partially with xanthine oxidase mediated lipid peroxidation.

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Inhibition of Xanthine Oxidase by Seaweed Extracts (해조류 추출물의 Xanthine Oxidase 저해작용)

  • 김외경;이태기;박영범;박덕천;이용우;여생규;김인수;박영호;김선봉
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.6
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    • pp.1069-1073
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    • 1996
  • Inhibition of xanthine oxidase by seaweed extracts obtained from Undaria pinnatifida, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum, Codiumfragile, Enteromorpha compressa and Porphyra tenera were investigated. Extracts of E. stolonifera and E. mua remarkably inhibited xanthine oxidase activity compared to those of other seaweed. The xanthine oxidase inhibitory activity of E. cava was higher than that of E. stolonifera. Diethyl ether extract from E. cava was more effective in the inhibition of xanthine oxidase than other solvent extracts. Two xanthine oxidase inhibitors(A-1 and A-2) from diethyl ether extract were isolated and purified by silica gel column chromatography, thin layer chromatography and high performance liquid chromatography. Xanthine oxidase inhibitory activities of these compounds were 27.8 and 48.1% per 0.4mg, respectively. The active compound A-2 had absorption peak at 420nm, 456nm and 467nm, which can be considered as siphonaxanthine.

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Studies on Xanthine Oxidase from Bovine Thyroid Glands -[Part 1] Purification and Substrate Specificity- (소의 갑상선에 있는 크산친 옥시다아제에 관한 연구 -[제1보] 효소의 정제와 기질특이성-)

  • Lee, Hyo-Sa
    • Applied Biological Chemistry
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    • v.21 no.2
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    • pp.112-118
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    • 1978
  • Xanthine oxidase from bovine thyroid glands was purified to apparent homogeneity when judged by analytical disc gel electrophoresis. The purification procedures include pancreatin digestion, butanol extraction, ammonium sulfate precipitation, calcium phosphate gel adsorption, ultrafiltration, calcium phosphate gel-cellulose column chromatography, gel filtration, preparative Sephadex G-25 column electrophoresis, and preparative polyacrylamide gel electrophoresis. The enzyme was enriched 1,000-fold. However, its specific activity was markedly low as compared with highly purified milk enzyme. Thyroidal xanthine oxidase exhibited a low specificity for substrates and electron acceptors. The kinetic properties of thyroid xanthine oxidase were found to be similar to those of the milk enzyme on the basis of Michaelis constants for common substrates.

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Inhibitory effect of medicinal plant extracts on xanthine oxidase activity (약용식물 추출물의 xanthine oxidase 활성 저해 효과)

  • Do, Jaeho;Gwak, Jungwon;Rho, Jung Jin;Lee, Kwangseung;Kim, Dong Chung
    • Journal of Applied Biological Chemistry
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    • v.62 no.3
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    • pp.275-280
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    • 2019
  • This study was performed to certify the inhibitory effect of aqueous extracts from sixty-seven medicinal plants on the activity of xanthine oxidase in vitro. Among the sixty-seven medicinal plants, Scutellaria baicalensis Georgi, Citrus aurantium L., Polygonum multiflorum Thunb., Pueraria thunbergiana (Sieb. et Zucc.) Benth., Citrus unshiu Marcor., Rubus coreanus Miquel, Camellia sinensis L., and Poncirus trifoliata Raf. were regarded as effective anti-gout sources. The active substances of P. multiflorum root extract were very stable at pH 2.0 and high temperatures. Xanthine oxidase activity was proportionally inhibited when concentrations of P. multiflorum extract increased. The aqueous extract from P. multiflorum root at a concentration of 2.0 mg/0.1 mL inhibited xanthine oxidase by 73.8%.

Inhibitory Effects of Xanthine Oxidase by Boiled Water Extract and Tannin from Persimmon Leaves (감잎 열탕 추출물 및 감잎 탄닌의 Xanthine Oxidase 저해 효과)

  • 문숙희;이민경
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.354-358
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    • 1998
  • The influence of hot water extracts and tannin obtained from persimmon leaves on xanthine oxidase were investigated. Above two samples had higher inhibitory effect against xanthine oxidase by hot water extracts and tannin obtained from persimmon leaves was 92.4% and 92.1% by addition of 2.0 mg/$m\ell$ of the hot water extracts and the tannin, respectively. The inhibitions by the hot water extracts and the tannin were of competitive mode with respect to xanthine as a substrate.

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Inhibitory Effects of the Solvent Fractions from Persimmon Leaves on Xanthine Oxidise Activity (용매별 감잎 추출물의 Xanthine Oxidase 저해효과)

  • 문숙희;이민경;채기수
    • The Korean Journal of Food And Nutrition
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    • v.14 no.2
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    • pp.120-125
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    • 2001
  • The inhibitory effects of xanthine oxidase by the methanol extract and the solvent fractions obtained from persimmon leaves were investigated. The inhibition ratio of xanthine oxidase was 78% by addition of 2.0mg/ml of the methanol extract. Among the solvent fractions, the ethylacetate fraction showed the strongest inhibitory effect against the xanthine oxidase, followed by the hexane fraction. The effect increased with addition of the ethylacetate fraction. At a concentration of 2.0mg/ml of the ethylacetate fraction, 65% of the enzyme activity decreased within 1.0 min of incubation with xanthine oxidase. But the activity of xanthine oxidise did not decrease significantly by the length of the incubation time.

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