한국콘텐츠학회논문지 (The Journal of the Korea Contents Association)

  • 제10권3호
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  • Pages.196-203
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  • 2010
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  • 1598-4877(pISSN)
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  • 2508-6723(eISSN)
한국콘텐츠학회 (The Korea Contents Association)
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미세전류가 토끼 경골의 골절 후 BMP-4 발현에 미치는 영향

The Effect of Microcurrent Stimulation on Expression of BMP-4 After Tibia Fracture in Rabbits

  • 투고 : 2009.08.26
  • 심사 : 2009.10.21
  • 발행 : 2010.03.28
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초록

본 연구는 BMP-4의 발현을 통해 골절 후 골유합에 대한 미세전류의 효과를 관찰하였다. 실험동물은 체중 2.5~3 kg내외의 6개월 령 뉴질랜드 웅성 토끼 24마리를 사용하였으며 경골 골절 후 미세전류를 적용한 실험군과 비적용군인 대조군으로 나누었고, 시간경과에 따른 변화를 관찰하기 위하여 3일, 7일, 14일 및 28일군으로 나누어 BMP-4에 대한 면역조직화학적 염색을 실행하여 다음과 같은 결론을 얻었다. BMP-4의 발현은 미세전류를 적용한 실험군과 자연치유군인 대조군 모두 시간이 경과함에 따라 증가하다가 감소되었다. 그러나 골절 7일 후 까지 동일 시점에서 실험군이 대조군에 비해 더욱 강한 면역양성 반응을 보였다. 특히 경골 골절 7일 후 대조군은 하버씨계의 동심원과 간질층판을 중심으로 중등도의 갈색의 면역양성반응을 보인데 반해 실험군의 경우 바깥층판을 포함하여 매우 강한 갈색의 면역양성반응을 보였다. 위의 결과로 보면 골절 후 미세전류를 적용할 때 치유과정 초기에 골형성단백질인 BMP-4의 발현을 증가시켜 골절 치유를 촉진시킴을 알 수 있었다.

This study aimed to examine the effect of microcurrent stimulation on expression of Bone Morphogenetic Protein(BMP) 4 after tibia fracture in rabbits. The twenty four adult 6 month old New Zealand white rabbits weighting 2.5~3.5 ㎏ were used. Twenty four rabbits with tibia fracture were randomly divided into control and experimental groups. Each group was divided into four subgroups, based on the duration of the experiment (3, 7, 14, 28 days). The experimental groups received microcurrent stimulation of 20~25 ${\mu}A$ intensity with surface Ag-AgCl electrode (diameter 1cm, Biopac, U.S.A.) for 24 hours a day. Cathode of the microcurrent stimulator located on the tibia directly, anode of it did on the gastrocnemius muscle. After evaluation, the test results are as follows: Comparisons of immunohistochemical observation of BMP-4 in 7 days after tibial fracture show that there was shown to be a moderate positive reaction (++) on concentric circles of Harversian system and the interstitial lamella in the control group, while there was a very strong positive reaction () on concentric circles of Harversian system and interstitial lamellain the experimental group. These results suggest that applying non-invasive constant microcurrent stimulation on fractured bone is helpful to bone healing.

키워드

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피인용 문헌

  1. Effects of Microcurrent and High Voltage Pulsed Galvanic Current Stimulation on Fibular Fracture Healing of the Rabbits vol.11, pp.10, 2011, https://doi.org/10.5392/JKCA.2011.11.10.286
  2. The Effects of Microcurrent Stimulation on Erector Spinae Fatigue Index vol.11, pp.11, 2013, https://doi.org/10.14400/JDPM.2013.11.11.545
  3. Effects of low-frequency electrical stimulation on cumulative fatigue and muscle tone of the erector spinae vol.27, pp.1, 2015, https://doi.org/10.1589/jpts.27.105