The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지

Effect of Phellinus igniarius Quel Extract on the Anti-inflammatory, Anti allergy, Anti-oxidant, Anti-wrinkle

상황버섯 추출물이 항염증 및 항알레르기, 항산화, 주름제거에 미치는 실험적 연구

  • 윤우식 (대전대학교 한의과대학 한방안이비인후피부과교실) ;
  • 정현아 (대전대학교 한의과대학 한방안이비인후피부과교실) ;
  • 노석선 (대전대학교 한의과대학 한방안이비인후피부과교실)
  • Received : 2010.03.11
  • Accepted : 2010.04.07
  • Published : 2010.04.25
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Abstract

Objectives : This study was carried out to investigate the effects of Phellinus igniarius Quel extract on the Anti-inflammatory, Anti allergy, Anti-oxidant and Anti-wrinkle. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH method. In the next experiment, to investigate anti-inflammatory test, the RAW 264.7 macrophage cells was cultured using DMEM including the 10% FBS. To study anti-allergic effect, we blended cultured Human Mast Cells(HMC-1), and then observe TNF-$\alpha$, IL-8 by ELISA Results : Phellinus igniarius Quel extract has the effects of anti-inflammation and anti-allergy, which may be due to its inhibitory potential on the macrophage activation. Furthermore, Phellinus igniarius Quel extract has the anti-wrinkle effects through the inhibitory potential on the collagnease, elastase and gelatinase activities. Conclusions : The above results suggest that Phellinus igniarius Quel extract could be applicable for improvement of several skin functions.

Keywords

References

  1. 盧石善. 原色皮膚科學. 서울: (주) 아이비씨기획. 2006:11-2, 111-21, 653, 739.
  2. 대한병리학회. 병리학. 서울: 고문사. 1995:71-104.
  3. 許 浚. 東醫寶鑑. 서울: 대한 형상 의학회. 2005:725-726.
  4. 안덕균. 한국본초도감. 교학사. 2003:549.
  5. 李娟熹. 발아현미상황버섯의 피부면역질환에 대한 영향(Effects of Phellinus linteus grown in germinated brown rice on skin immune disorders). 서울: 建國大學校. 2006.
  6. 金泰勳. 상황버섯(Phellinus baumi)추출물의 항산화 작용과 자유라디칼의 소거효과. 진주:慶尙大學校. 2002.
  7. 孟垠鎬. 桑黃버섯(Phellinus linteus) 子實體分泌物의 免疫活性 및 항암효과. 서울: 단국대학교. 2001.
  8. 안성민. 상황버섯 균사체 인체 간암세포와 흰쥐의 간에서 염증관련 단백질에 미치는 효과. 부산대 대학원. 2005.
  9. 천순주. 상황버섯(Phellinus linteus)의 화장품 약리 활성검증과 한방화장품 응용에 관한 연구. 경산, 대구한의대학교. 2007.
  10. Wadsworth T.L., Koop D.R., Effects of Ginkgo biloba extract and quercetin on lipopolysaccharide-induced release of nitric oxide. Chem. Biol. Interact. 2001;137:43-58. https://doi.org/10.1016/S0009-2797(01)00208-3
  11. Hinz B., Brune K., Rau T., Pahl A., Flurbiprofen enantiomers inhibit inducible nitric oxide synthase expression in RAW 264.7 macrophages. Pharm. Res. 2001;18:151-6. https://doi.org/10.1023/A:1011020132140
  12. Amersham, Manual for Prostaglandin E2 Biotrak Enzymeimmunoassay (EIA) system.
  13. Chomczynski P., Sacchi N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156-9.
  14. Biosource, Manual for TNF-${\alpha}$ ELISA KAP-1751.
  15. Biosource, Manual for IL-6 ELISA KAP=1261.
  16. Biosource, Manual for TNF-${\alpha}$ ELISA KAP-1751.
  17. Kim C.D., Lee W.K., No K.O., Park S.K., Lee M.H., Lim S.R., Roh S.S., Anti-allergic action of buckwheat (Fagopyrum esculentum Moench) grain extract. Int Immunopharmacol. 2003;3:129-136. https://doi.org/10.1016/S1567-5769(02)00261-8
  18. Jung M.Y., Thapa N., Kim J.E., Yang J.D., Cho B.C., Kim I.S., Recombinant tetra-cell adhesion motifs supports adhesion, migration and proliferation of keratinocytes/fibroblasts, and promotes wound healing. Exp Mol Med. 2007;39:663-672. https://doi.org/10.1038/emm.2007.72
  19. Invitrogrogen, Manual for EnzChek${\circledR}$ Gelatinase/Collagenase Assay Kit E-12055.
  20. Invitrogrogen, Manual for EnzChek${\circledR}$ Elastase Assay Kit E-12056.
  21. Blos M.S., Antioxidant determination by the use of stable free radical. Nature. 1958;181:1199. https://doi.org/10.1038/1811199a0
  22. Denizot F., Lang R., Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Immunol.Methods. 1986;89: 271-7. https://doi.org/10.1016/0022-1759(86)90368-6
  23. 주영승. 운곡본초학 각론. 서림재, 2004:50-7.
  24. 陳實功. 外科正宗. 서울: 天津科學技術出版社. 1993:47.
  25. 楊維傑. 黃帝內經解釋. 서울: 成輔社. 1997:147, 210, 299, 349, 456.
  26. 華 陀. 中藏經. 서울: 成輔社 1997:82.
  27. 王 燾. 外臺秘要. 北京: 人民衛生出版社. 1982:625-31,107.
  28. 嚴用和. 濟生方. 서울: 東洋醫藥大學. 1965:61-68.
  29. 危亦林. 得效方. 서울: 東洋醫藥大學. 1965:613.
  30. 박광균. 구강생화학. 서울: 군자출판사. 1999:318-325.
  31. 약품생화학분과위원회편. 분자약품생화학. 서울: 동명사. 2003:59-71.
  32. 김륭규, 신경민, 천상국, 지사영, 서성훈, 박희준, 최종원, 이경태. 넙취 정유의 murine macrophage Raw 264.7 세포에서의 in vitro 항암효과. 약학회지. 2002;46(5):343-9.
  33. 정해영역, Toshio Hirano편저. 생명과학과 분자의학을 위한 분자생물학. 서울; 월드 사이언스. 2002:73-85.
  34. 노민수, 하준용, 이창훈, 이우영, 이수환, 이정준. Inhibitory activities of natural products on lipopolysaccharide induced prostaglandin production in mouse macrophages. 약학회지, 1998;42(6):558-566.
  35. 서영준. 발암과정에 있어서 cyclooxigenase-2 의 역할 및 그 저해를 통한 화학 암예방. 분자세포생물학뉴스, 2001;13:8-17.
  36. 張民慶. 中醫皮膚美容方劑大全. 北京; 중국중의약출판사. 2001:34.
  37. 강원형. 피부질환 아틀라스, 서울; 도서출판 한미의학. 2003:259-60,289-93,337-40,369-71,381-4.
  38. 김경동. 한방 천연식물의 항산화 및 자외선 흡수에 관한 연구. 한국미용학회지. 2004;10(2):145-53.
  39. Jung JH., Photoaging. Photodermatol Photoimmunol Photomed. 2003;19:109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x