Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Development of Anti-Wrinkle Materials using Galloyl-Peptide Derivatives

갈릭산 펩타이드 유도체를 이용한 주름개선 소재 개발

  • 정해수 ((주)바이오에프디엔씨 항노화연구소) ;
  • 송미영 ((주)바이오에프디엔씨 항노화연구소) ;
  • 김형식 ((주)바이오에프디엔씨 항노화연구소) ;
  • 서효현 ((주)바이오에프디엔씨 항노화연구소) ;
  • 이정훈 ((주)바이오에프디엔씨 항노화연구소) ;
  • 이경록 ((주)아미코스메틱) ;
  • 홍일 ((주)아미코스메틱) ;
  • 모상현 ((주)바이오에프디엔씨 항노화연구소)
  • Received : 2015.05.15
  • Accepted : 2015.08.06
  • Published : 2015.08.31
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Abstract

Conjugating a phytochemical, a strong antioxidant, with a functional peptide not only compensates for its stability, but also improves its solubility and anti-wrinkle effects, thereby contributing to the possibility of becoming an excellent cosmetic ingredient. Thus, in this study we examined the potential cosmetic use of a phytochemical-peptide derivative using gallic acid, a phytochemical with antioxidant, anti-inflammatory, and anti-cancer effects. To evaluate the antioxidant and wrinkle-improving efficacy of 5 synthesized gallic acids conjugated with LVH, IVH, KTTKS, YGGFM, and YGGFLRKYP respectively, we observed the expression of genes related to wrinkle improvement using DPPH radical scavenging activity and real-time PCR. As a result, all 5 derivatives had excellent free radical scavenging effects. The expression level of genes involved collagen synthesis also increased, and the secreted peptides during collagen production contributed to their antioxidant and wrinkle improving effects. These results mark the potential use of gallic acid peptide derivatives as a cosmetic ingredient for anti-oxidation and wrinkle improvement.

항산화 효능이 우수한 phytochemical에 콜라겐 합성능이 우수한 기능성 펩타이드의 결합을 통해 소재 자체의 안정성뿐만 아니라 수용액 상태에서의 분산성을 증대시켜 주름개선 효능과 더불어 우수한 화장품 소재로서의 가능성을 가진다. 이에 본 연구는 항산화, 항염 및 항암 등의 효능을 가진다고 알려져 있는 phytochemical인 갈릭산을 이용해 phytochemical-펩타이드 유도체를 개발하여 화장품 소재로서의 가능성을 시험하였다. 갈릭산에 LVH, IVH, KTTKS, YGGFM, YGGFLRKYP 총 5종의 펩타이드를 각각 합성하여 만든 갈릭산 펩타이드 유도체의 항산화 및 주름개선의 효능을 평가하고자 DPPH radical scavenging activity와 real-time PCR로 주름개선과 관련된 유전자의 발현을 확인하였다. 그 결과 5종 유도체 모두 우수한 자유 라디컬 소거능을 보였다. 또한 collagen 합성에 관여하는 유전자의 발현이 증가하였고, collagen 생성시 분비되는 펩타이드의 증가를 통해 항산화뿐만 아니라 주름개선에도 효과가 있었다. 이를 통해 갈릭산 펩타이드 유도체가 항산화 및 주름개선을 위한 화장품 소재로서의 가능성을 확인하였다.

Keywords

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