Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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In vivo Toxicity and Immunoadjuvant Activity of Korean Mistletoe (Viscum album coloratum) Extract Fermented with Lactobacillus

한국산 겨우살이 유산균 발효 추출물의 독성 및 면역증강 효과

  • Yoon, Taek-Joon (Deptartment of Food & Nutrition, Yuhan University) ;
  • Yang, Woong-Suk (Institute for Biomedical Research, Han-Dong University) ;
  • Park, Sung-Min (Institute for Biomedical Research, Han-Dong University) ;
  • Jung, Hoe-Yune (Institute for Biomedical Research, Han-Dong University) ;
  • Lee, An-Na (Institute for Biomedical Research, Han-Dong University) ;
  • Jung, Jin-Hyuk (Institute for Biomedical Research, Han-Dong University) ;
  • Kang, Tae-Bong (Institute for Biomedical Research, Han-Dong University) ;
  • Yoo, Yung-Choon (Institute for Biomedical Research, Han-Dong University) ;
  • Kim, Jong-Bae (Institute for Biomedical Research, Han-Dong University)
  • 윤택준 (유한대 식품영양과) ;
  • 양웅석 (한동대학교 생의학연구소) ;
  • 박성민 (한동대학교 생의학연구소) ;
  • 정회윤 (한동대학교 생의학연구소) ;
  • 이안나 (한동대학교 생의학연구소) ;
  • 정진혁 (한동대학교 생의학연구소) ;
  • 강태봉 (한동대학교 생의학연구소) ;
  • 유영춘 (한동대학교 생의학연구소) ;
  • 김종배 (한동대학교 생의학연구소)
  • Published : 2009.10.31
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Abstract

In this study, Korean mistletoe extract (KM-110) was fermented with two strains of Lactobacillus (FKM-110) and then toxicity, lectin content, and immune activities were investigated. The lectin content of FKM-110 was about 53-71% lower than that of KM-110. When mice were subcutaneously administered with KM-110 and FKM-110, the $LD_{50}$ obtained for KM-110 treatment was 50-100 mg/kg as compared to 150-200 mg/mL for FKM-110. Each preparation stimulated macrophages directly and enhanced productivity of inflammatory cytokines such as TNF-${\alpha}$ and IL-$1{\beta}$. FKM-110 treatment resulted in lower cytokine production compared to KM-110. When mice were immunized with Keyhol limpet hemocyanin (KLH) antigen along with KM-110 or FKM-110 administration, higher antibody titers to KLH were observed in the KM-110 or FKM-110 groups compared to mice immunized with KLH alone, thereby showing no difference between KM-110 and FKM-110. Therefore, fermentation of Korean mistletoe extract with these Lactobacillus strains decreased toxicity in vivo while the enhancement of immune activity by KM-110 and FKM-110 was similar. These data suggest that KM-110 fermentation tended to decrease lectin content and in vivo toxicity. In addition, other components in the fermented mistletoe extract appear to stimulate immuno-adjuvant activity instead of lectin.

Lactobacillus casei KCTC 2180 및 Lact. plantarum KCTC 3103으로 발효된 한국산 겨우살이 추출물인 FKM-110의 렉틴 성분의 함량 변화, 독성 및 면역증강 효과를 검토하여 다음과 같은 결과를 얻었다. 두 가지 유산균을 이용하여 KM-110을 1-3일간 발효시킨 결과, 렉틴 성분은 발효 전에 비하여 53-71% 정도의 함량을 나타냄으로서 감소되는 경향을 보였다. 마우스에 대한 시료의 독성시험을 위하여 각 시료를 피하 주사하고 $LD_{50}$ 값을 측정한 결과, KM-110은 50-100 mg/kg인 반면 유산균으로 발효된 겨우살이 추출물인 FKM-110은 150-200 mg/kg인 결과를 보임으로 발효에 의하여 in vivo 독성효과는 감소하는 경향을 보였다. 각 시료를 macrphage에 직접 자극하여 염증성 cytokine인 IL-$1{\beta}$ 및 TNF-${\alpha}$의 생산능력을 조사한 결과, FKM-110이 KM-110에 비하여 낮은 cytokine의 생산양식을 나타냈다. 그러나 FKM-110의 항원 KLH에 대한 면역증가 효과를 항체 생산능으로 조사한 결과, KM-110과 차이 없이 항원 단독 투여군에 비하여 높은 역가의 항체가 생산되었다. 따라서 겨우살이 추출물을 유산균으로 발효시킨 결과, in vivo 독성은 감소하였으나 면역증강 활성은 KM-110과 동일하게 유지되는 결과를 얻었다. 따라서 겨우살이의 면역증강 활성에 관여하는 성분으로 렉틴 이외에 물질이 관여할 수 있음을 강하게 시사하였다.

Keywords

References

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