Most control systems of power plants are using classical PID controllers for their process control. In order to get the desired control performances, the correct tuning of PID controllers is very important. Sometimes, it is necessary to retune PID controllers after the change of system operating condition and system design change, etc. Commercial auto-tuning controllers such as relay feedback controller can be used for this purpose. However, using these controllers to the safety-critical systems of nuclear power plants may be cause of unsafe operation, because they are using test signals for tuning. A new system identification auto-tuning method without using test signal has been developed in this paper. This method uses process input/output signals for system identification of unknown control process. From the model information of control process which was obtained from system identification approach, the optimal PID parameters can be calculated. The method can be used in the safety-critical systems because it is not using test signals during system modeling process.