Fabrication of 2-D Microwell Arrays for Micro ELISA System

초소형 면역 분석기용 이차원적 배열형 마이크로 우물 제작

  • Lee, Kook-Nyung (School of Electrical Engineering, Seoul National University) ;
  • Ahn, Si-Hong (School of Electrical Engineering, Seoul National University) ;
  • Park, Jae-Hyung (School of Electrical Engineering, Seoul National University) ;
  • Kim, Yong-Kweon (School of Electrical Engineering, Seoul National University)
  • Published : 1998.07.20

Abstract

2-D microwell arrays for micro ELISA (Enzyme-Linked Immuno Solvent Assay) system were fabricated using micromachining technology. The materials for the bottom plate, top plate and sidewall of the microwell were used a #7740 glass, gold and silicon respectively considering bio-compatibility and easy fabrication. Cylindrical or groove shape microwells, which have about $100{\mu}m$ depth and $50{\sim}500{\mu}m$ diameter or width, were arrayed. The fabricated microwell array can be applied to the essential part of a biochip when surface modification is made to immobilize cells or biomolecules on the microwell bottom.

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