Evaluation of Activated Platelet Using Peptide-Immobilized Surface

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RGDF immobilized micro-patterned surface was developed to detect the functional state of platelets. Using photolithographic technology, an RGDF micro-patterned surface was prepared on silicon wafer. Platelet adhesion to this surface was observed by fluorescence microscopy after staining platelets with mepacrine. Nonactivated platelets pretreated with $PGE_1$ interacted incompletely with the RGDF micro-patterned surface, whereas activated platelets treated with ADP interacted with the surface extensively. These results show that the distinct selectivity of an RGDF-immobilized micro-patterned surface can be used to detect the functional state of platelets.