Characterization of the recombinant metalloprotease from Vibro mimicus and its hemagglutinating activity

  • Published : 2000.05.01

Abstract

Metalloprotease produced in Vibrio mimicus, in which zinc is an essential meta ion for catalytic activity, degrades a variety of biologically important substances including human collagen, several complement components, and immunoglobulin. For gene overexpression and convenient purification, VMC gene was constructed in pET22b(+) expression vector by using of PCR. (omitted)

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