Effect of Glutamic Acid on Polysaccharide Production from Agaricus blazei in Liquid Culture

  • 황정민 (강원대학교 환경 생물공학부) ;
  • 임영수 (강원대학교 환경 생물공학부) ;
  • 권명상 (강원대학교 수의학과) ;
  • 최정우 (서강대학교 화학공학과) ;
  • 한진수 (삼성생명과학연구소) ;
  • 홍억기 (강원대학교 환경 생물공학부)
  • Published : 2000.04.08

Abstract

The polysaccharide has been known to have an antitumor activity, which were extracted from the fruiting bodies, mycelia, and culture broths of Agaricus blazei. For the cell growth and the polysaccharide production, the optimal medium contained 8% glucose, 2% yeast extract, 0.1% $MgSO_4{\cdot}7H_2O$, 0.2% $KH_2PO_4$, 0.2% $CaCl_2{\cdot}2H_2O$ and 0.2% $MnSO_4{\cdot}5H_2O$. When 0.2% of glutamic acid was added at 4day, the cell concentration was 13.5 g/L and the polysaccharide production was 9.9 g/L, respectively.

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