한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)
- 한국자원식물학회 2001년도 The 8th International Symposium
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- Pages.49-58
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- 2001
Expression of dirigent protein and Pinoresinol/Lariciresinol reductase genes of forsythia in transgenic potatoes.
- Chuong, Tran-Van (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB)) ;
- Kim, Hyunsoon (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB)) ;
- Park, Jiyoung (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB)) ;
- Jaeyoul Joung (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB)) ;
- Jungwon Youm (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB)) ;
- Jaeheung Jeon (Plant Cell Biotechnology Lab. Korean Institute of Bioscience and Biotechnology (KRIBB))
- 발행 : 2001.11.01
초록
We tried to introduce two forsythia genes related in lignan biosynthesis, dirigent protein and pinoresinol/lariciresinol (P/L) reductase, into potatoes for accumulation of lignans in transgenic potatoes. We made binary vectors overexpressing dirigent protein gene and P/L reductase gene driven by a CaMV35S promoter and transformed into potatoes via Agrobacterium mediated transformation. And in order to control the metabolic flux of lignan biosynthesis pathway, we tried to inhibit chalcone synthase genes of potatoes by antisense inhibition technique also. We tried to use PCR screening method for selection of transgenic plants of different vectors. We tried to determine and compare lignan contents from different transgenic potato lines.
키워드