Proceedings of the Korean Biophysical Society Conference (한국생물물리학회:학술대회논문집)

The Korean Biophysical Society (한국생물물리학회)
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Characterization of Protein Factor Regulating the Superoxide-Sensor SoxR in Escherichia coli

  • Koo, Mi-Sun (Lab. of Molecular Microbiology, School of Biological Science and Institute of Microbiology, Seoul National University) ;
  • Rah, So-Yeon (Lab. of Molecular Microbiology, School of Biological Science and Institute of Microbiology, Seoul National University) ;
  • Lee, Kang-Lok (Lab. of Molecular Microbiology, School of Biological Science and Institute of Microbiology, Seoul National University) ;
  • Roe, Jung-Hye (Lab. of Molecular Microbiology, School of Biological Science and Institute of Microbiology, Seoul National University)
  • Published : 2001.06.01
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Abstract

Escherichia coli has developed soxRS regulon to defend against toxicity of superoxide radical. SoxR, superoxide sensor, is oxidized by superoxide-generating agents or nitric oxide and oxidized SoxR activates the transcription of soxS gene. In order to find out the trans-acting factors regulating SoxR activity in vivo, soxS：：lacZ single copy operon fusion construct was prepared and random Tn10 insertional mutatons were performed.(omitted)

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