한국생물공학회:학술대회논문집
- 2001.11a
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- Pages.70-73
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- 2001
Metabolic engineering for isoprenoids production in Escherichia coli
- Kim, Seon-Won ;
- Keasling, J.D. (Dept. of Chemical Engineering UC Berkeley)
- 김선원 (경상대학교 자연과학대학 식품영양학과) ;
- Published : 2001.11.07
Abstract
Isopentenyl diphosphate (IPP) is the common, five-carbon building block in the biosynthesis of all isoprenoids. IPP in Escherichia coli is synthesized through the non-mevalonate pathway. The first reaction of IPP biosynthesis in E. coli is the formation of 1-deoxy-D-xylulose-5-phosphate(DXP), catalyzed by DXP synthase and encoded by dxs. The second reaction in the pathway is the reduction of DXP to 2-C-methyl-D-erythritol-4-phosphate, catalyzed by DXP reductoismerase and encoded by dxr. To determine if one of more of the reactions in the non-mevalonate pathway controlled flux to IPP, dxs and dxr were placed on several expression vectors under the control of three different promoters and transformed into three E. coli strains (
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