한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Biosynthesis of Carbohydrate associated with secondary Metabolites; Biosynthesis of dTDP-4-keto-6-deoxy-D-glucose by four enzyme reaction system

  • 오종민 (서울대학교 응용화학부 유전공학연구소 생물공학실험실) ;
  • 이선구 (서울대학교 응용화학부 유전공학연구소 생물공학실험실) ;
  • 김병기 (서울대학교 응용화학부 유전공학연구소 생물공학실험실)
  • Published : 2001.11.07
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Abstract

We have developed a synthetic method for dTDP-4-keto-6-deoxy-D-glucose with four enzyme system. We have used crude extracts from cultures of Escherichia coli BL21 strains harboring plasmids containing different sources. dTDP-4-keto-6-deoxy-D-glucose was synthesized by the combination of thymidine-monophosphate kinase, acetate kinase, dTDP-glucose synthase and dTDP-D-glucose 4,6-dehydratase in a batch system, starting the reaction with dTMP. The enzymatic synthesis strategy allowed a dTMP conversion with a 95%.

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