Effect of specific growth rate on the extracellular expression of Baccillus stearothermophillus Ll lipase in recombinant Saccharomyces cerevisiae

Recombinant lipase gene (pYEGA ${\alpha}$ -lip) originated from Bacillus stearothermophillus Ll was overexpressed in Saccharomyces cerevisiae. The lipase gene expression level was compared by controlling a constant specifjc growth rates( ${\mu}$ = 0.03, 0.05, 0.07 and $0.1h^{-1}$. Cell g개wth was successfully controlled at the desired rates by feeding rate of glucose and the formation of by-product or accumulation of the glucose was not observed. Above the growth rate of $0.1h^{-1}$. the desired growth rate could not be achieved caused accumulation of by-products(ethanol). The lipase production increased as the specific growth rate decreased. The specific production rate at the lowest specific growth rater(${\mu}$ =0.03) was above 2- folds than the others.