한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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A study of in situ immobilization of lipase by using an LC column with aldehyde-silica stationary phase

  • 서우용 (명지대학교 환경생명공학과) ;
  • 홍선희 (명지대학교 환경생명공학과) ;
  • 이기세 (명지대학교 환경생명공학과)
  • Published : 2001.11.07
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Abstract

The in situ immobilization of lipase in an LC column with aldehyde-silica stationary phase was optimized. The effects of feed composition, temperature, mobile phase pH and now rate on immobilization efficiency were investigated. Lipase activity after in situ immobilization was compared with free enzyme and batch immobilized enzyme.

Porcine pancrease lipase를 aldehyde-silica에 in situ 상태에서 immobilization 할때의 최적 조건에 대하여 연구하였다. Feed 용액의 조성비율, 양, pH, 온도, 농도, 유속에 대하여 최적화하였으며 lipase의 activity를 측정 비교하였다.

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