Aspergillus niger LK 유래의 epoxide hydrolase 클로닝 및 특성 분석

  • 이은정 (경성대학교 응용공학부 식품공학과) ;
  • 김초희 (경성대학교 응용공학부 식품공학과) ;
  • 송성광 (경성대학교 응용공학부 식품공학과) ;
  • 김희숙 (경성대학교 응용공학부 식품공학과) ;
  • 이은열 (경성대학교 응용공학부 식품공학과)
  • Published : 2001.11.07

Abstract

Kinetic resolution of various racemic aromatic epoxides by newly isolated Aspergillus niger LK has been investigated, and enantioselectivity of whole-cell biocatalyst was analyzed. The epoxide hydrolase (EHase) of A. niger LK was cloned using RT-PCR. The sequence homology was compared with that of other microbial EHase and the gene for EHase was characterized at molecular level.

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