한국생물공학회:학술대회논문집
- 2002.04a
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- Pages.281-284
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- 2002
Production of Newcastle vaccine using continuous mammalian cells
- Gwak, Il-Yeong (Graduate School of Biotechnology, Korea University) ;
- Choe, Yeon-Suk (Graduate School of Biotechnology, Korea University) ;
- Jeong, Yeon-Ho (Division of Biotechnology, College of Agriculture and Life Science, Kangwon National University) ;
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Jeon, Gye-Taek
(Division of Life Science, College of Natural Sciences, Kangwon National University)
;
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Kim, Ik-Hwan
(Graduate School of Biotechnology, Korea University)
- Published : 2002.04.12
Abstract
Specific pathogen free (SPF) eggs have been used to produce live vaccines. however, their application causes many problems such as cost, space and waste disposal. The substitution of mammalian cells for SPF eggs offers a desirable system of vaccine production. In this study, mammalian cells were tested for the infection of Newcastle disease virus (NDV). As a result, DF-I and MDBK cells showed high virus productivity compared to the other mammalian cells. For the highest productivity of NDV, the optimal multiplicity of infection (M.O.I.) in DF-I or MDBK cells was determined to be 0.2 or 0.5 M.O.I., respectively.
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