Opsonized Streptococcus mutans influenced maturation of human dendritic cells

  • Ida, N. (Tokushima University) ;
  • Ozaki, K. (Tokushima University) ;
  • Suenobu, S. (Tokushima University) ;
  • Takashi, K. (Tokushima University) ;
  • Yamaguchi, D. (Tokushima University) ;
  • Matsuo, T. (Tokushima University)
  • Published : 2003.11.01

Abstract

I. Objectives It is reported that there are complements and immunogloblins in serum from dental pulp in dentinal tubules, and it is thought that dental caries bacteria is opsonized by these serum ingredients, and it is presented by dendritic cells(DCs) in dental pulp. So, we examined whether a maturational difference of DCs occured when S. mutans was opsonized. II. Materials and Methods PBMC was divided from normal human peripheral blood and collected CD14 positive cells by magnetic beads system. Adherent cells were incubated in 5% FCS-RPMI medium included GM-CSF, IL-4 for seven days.(omitted)

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