# Morphological and functional changes of dissociated single pancreatic acinar cells: testing the single cell as a model for exocytosis and calcium signaling

• Lee, Misun (Department of Physiology, Sungkyunkwan University School of Medicine) ;
• Uhm, Dae-Yong (Department of Physiology, Sungkyunkwan University School of Medicine) ;
• Park, Myoung-Kyu (Department of Physiology, Sungkyunkwan University School of Medicine)
• Published : 2003.06.01

#### Abstract

Isolated single pancreatic acinar cells have long been used as a good model for studying many kinds of signaling processes due to their good structural and functional polarities without a significant validation. In this study, we have examined morphological and functional changes of the dissociated single pancreatic acinar cells by imaging cytosolic Ca$\^$2+/ concentration, exocytosis of granules, and by observing their shapes with confocal microscopy.