Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
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- Pages.136.2-137
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- 2003
Phytoplasma specific primer for detection of jujube witches′ broom group(16SrV) in Korea and China
- Sangsub Han (Dept. of Agricultural Biology, Chungbuk National University) ;
- Lee, Sanghun (Dept. of Agricultural Biology, Chungbuk National University) ;
- Mengjun Liu (Research Center of Chinese Jujube, Agricultural University) ;
- Byeongjin Cha (Dept. of Agricultural Biology, Chungbuk National University)
- Published : 2003.10.01
Abstract
In order to diagnose and differentiate jujube witches' broom (JWB) phytoplasma rapidly, oligonucleotide primer pair, 16Sr(V) F/R, for polymerase chain reactions (PCRs) was designed on the basis of 165 rRNA sequences of JWB phytoplasma. The PCR employing phytoplasma universal primer pair P1/P7 consistently amplified DNA in all tested phytoplasma isolates. But no phytoplasma DNA was detected in healthy jujube seedlings. The nested PCR, the primer pair 16S(V) F/R, about 460 bp fragment, amplified DNA in all tested JWB and related phytoplasmas including LiWB phytoplasma of the 165 rRNA group V, but no DNA amplification was detected from other phytoplasma strains such as group 16SrI (Aster yellows) and group 16SrⅩII (Stolbur group) phytoplasmas in which mulberry dwarf phytoplasma and chrysanthemum witches broom phytoplasma are belonged to, respectively The same results were obtained from both Korean- and Chinese-isolates of JWB. Nested-PCR using phytoplasma universal primer pair P1/P7 and 16S rRNA group V specific primer pair 16S(V) F/R could detect group V phytoplasma rapidly and easily, in particular JWB phytoplasma.
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