Proceedings of the SCSK Conference (대한화장품학회:학술대회논문집)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

EFFICACY EVALUATION OF THE WHITENING COSMETICS USING IN VITRO TYROSINASE INHIBITION ASSAY

  • Lee, J. P. (Drug Evaluation Department, Korea Food and Drug Administration) ;
  • Kim, Y. O. (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • J. Y. Jang (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • K. H. Son (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • S. J. Yang (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • Lee, K. S. (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • Kim, W. H. (Drug Evaluation Department, Korea Food and Drug Administratio) ;
  • J. T. Hong (College of Pharmacy, Chungbuk National Universit) ;
  • Park, S. S. (Drug Evaluation Department, Korea Food and Drug Administration)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the tyrosinase inhibitory effect using whitening materials such as arbutin, ethyl ascorbyl ether, glabridin, kojic acid, magnesium ascorbyl phosphate and ascorbic acid. Tyrosinase inhibition rate were determined varying the enzyme concentration, reaction time, reaction temperature and pH. The optimal conditions to measure the inhibitory efficacy were as follows. : enzyme concentration 1,500 or 2,000IU/mL, reaction time 15min(for the enzyme concentration 1,500 IU/mL) and l0min(for the enzyme concentration 2,000IU/mL), reation temperature 42$^{\circ}C$, pH 6.5. Under these conditions $IC_{50}$/ of arbutin, ethyl ascorbyl ether, glabridin, kojic acid, magnesium ascorbyl phosphate and ascorbic acid were calculated. In the case of magnesium ascorbyl phosphate, the inhibitory effect of tyrosinase was very low and the $IC_{50}$/ of magnesium ascorbyl phosphate could not be calculated. Other five materials showed good inhibitory effect of tyrosinase and can be used for the whitening materials.

Keywords