High throughput approaches to predicting drug absorption potential using the immobilized artificial membrane phosphatidylcholine column and molar volume

The purpose of this study was to evaluate the predictability of the fraction of drug absorbed in humans using the immobilized artificial membrane phosphatidylcholine column (IAMPC) under optimized conditions in comparison with a conventional IAMPC method. Twenty commercial drugs, both acidic and basic in nature, were used in the study, Drugs were dissolved in acetonitrile:water (50:50, v/v) at a concentration of 100 mg/ml, and were injected on HPLC/UVD at a mobile phase (acetonitrile:DPBS = 10:90,v/v) with a flow rate of 0.5 ml/min equilibrated at 37$^{\circ}C$. (omitted)