Studies on Optimum Conditions for Polysaccharide Production from Schizophyllum commune in flask Culture

Schizophyllum commune belongs to Basidiomycetes is able to produce an extracellular polysaccharide, beta-glucan, which has been well known as an effective immune stimulator. In other to obtain the optimum conditions for the polysaccharide production, Schizophyllum commune was cultivated in different cultivation media containing various carbon and nitrogen sources. For the cell growth and the polysaccharide production, the optimum synthetic medium was developed with YMP medium an a basal midium. The flask culture conditions for the polysaccharide production were $27^{\circ}C$ and 150rpm with the initial pH 5.5.

Schizophyllum commune의 균체 생육 및 다당체 생성에 미치는 여러 가지 배지성분의 영향을 조사하였다. 탄소원으로는 산업적인 경제성과 높은 다당체 생성량을 나타낸 glucose를 선택하였고, 무기질소원은 유기질소원에 비해 현저히 낮은 다당체 생성량을 나타내었으며, 그 결과 균체량과 다당체 생성량에서 높은 효과를 나타낸 yeast extract를 질소원으로 선택하였다.