Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference (한국식품저장유통학회:학술대회논문집)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Separating and purifying polysaccharide, polypeptide from sea cucumber and their functions

해삼으로부터 다당류와 폴리펩타이드의 분리, 정제 및 기능성

  • Jin, Hai-zhu (Institute of Food Science and Engineering, Yanti University) ;
  • Fu, Xue-jun (Institute of Food Science and Engineering, Yanti University) ;
  • Shen, Jing-yu (Institute of Food Science and Engineering, Yanti University) ;
  • Sun, Bo (Institute of Food Science and Engineering, Yanti University) ;
  • Wang, Hong-tao (Institute of Food Science and Engineering, Yanti University)
  • Published : 2005.09.01
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Abstract

For thestudying and utilizing the thorn sea cucumber synthetically, the method preparing polysaccharide and polypeptide from sea cucumber was studied. The comparatively rational craft for preparing crude polysaccharide is fresh raw materials deal with oar form, $60\%$ of the ethanol precipitate after hydrolyzed by pretense and vibrated with ultrasonic wave auxiliary. The purification of polysaccharide and removal of protein fromcrude polysaccharide were made through precipitation method using the acetate. The polypeptide is obtained by concentrating in vacuum, freeze-drying after mixing precipitations of two times. Polysaccharide acute poisoning experiment indicate there is no bad reaction when LD50>5000g/kg, there strain rate of liver tumor H11 approach present generally acknowledged tumor treat medicine cyclophosphamide, and there is no side effect. The fatigue resistance function of polypeptide experiment also indicates that the fatigue resistance ability of mouse which fed on added sea cucumber polypeptide has a great improvement.

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