한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Immobilization of Phospholipase C by sol-gel method

  • Sohn, Ok-Jae (Department of Materal and Biochemical Engineering, BioProcess Technology lab., Chonnam National University) ;
  • Yim, Yong-Sik (BioProcess Technology lab., Chonnam National University) ;
  • Jung, Jin-Su (Faculty of Applied Chemical Engineering) ;
  • Lee, Yong-Jun (Faculty of Applied Chemical Engineering) ;
  • Lee, Hye-Na (Faculty of Applied Chemical Engineering) ;
  • Rhee, Jong-Il (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Chonnam National University)
  • Published : 2005.04.15
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Abstract

Phospholipases C (PLCs) from B. cereus 318 and recombinant Pichia pastoris were immobilized on sol-gel coated glass beads. The pH and temperature on immobilized PLC activity were investigated. Operational and storage stability of the immobilized PLCs was measured by spectro- photometric assay. The PLCs immobilized on sol-gel coated glass beads were photographed by scanning electron microscope (SEM).

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