Optimization of Cultural Conditions for Mycelial Growth and Exo-Polysaccharide Production in Jar Fermentation by Fomitopsis pinicola

  • Cha, Wol-Suk (Dept. of Chemical Engineering, Chosun University) ;
  • Jilu, Ding (Dept. of Chemical Engineering, Chosun University) ;
  • Lee, Choon-Beom (Dept. of Chemical Engineering, Chosun University) ;
  • Nam, Hyung-Geun (Dept. of Chemical Engineering, Chosun University) ;
  • Lee, Jun-Han (Dept. of Chemical Engineering, Chosun University) ;
  • Maeng, Jeung-Moo (Dept. of Chemical Engineering, Chosun University) ;
  • Lim, Hwan-Hee (Dept. of Chemical Engineering, Chosun University)
  • Published : 2005.04.15

Abstract

The Study was carried out to investigate in the optimal mycelial growth and Exo-Polysaccharides of Fomitopsis pinicola. Jar fermentations were carried out to optimize the culture conditions for mycelial growth and exo- polysaccharide production. The optimal agitation speed and aeration rate were 200 rpm and 1.5 v.v.m., respectively. Under optimal culture conditions, the maximum mycelial growth and exo-polysaccharide production after 11 days with a 5 L jar fermenter containing the optimized medium were 10.21 g/L and 3.56 g/L, respectively. However, the fundamental information obtained this study is insufficient in the development of a efficient process for mycelial growth and exe-polysaccharide production from Fomitopsis pinicola.

발효기에서의 균사체 생장과 세포외다당류의 생산을 위한 최적 배양조건을 조사하기 위하여 회분식 반응기를 이용하여 연구한 결과 최적 회전속도와 통기량은 각각 200 rpm과 1.5 vvm 었다. 최적 배양 환경하에서 5L발효기(working volume 2 L)에서 배양한 결과 각각 10.21 g/L 와 3.56 g/L의 mycelial growth와 exo- polysaccharide를 얻었다.

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