한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development and culture optimization of mutants of Thiobacillus sp. IW for elimination of hydrogen sulfide

  • Kang, Sun-Chul (Division of Food, Biological & Chemical Engineering, Daegu University) ;
  • Lim, Kwang-Hee (Division of Food, Biological & Chemical Engineering, Daegu University) ;
  • Shin, Seung-Yong (Division of Food, Biological & Chemical Engineering, Daegu University)
  • Published : 2005.10.27
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Abstract

UIW-10 mutant obtained by UV treatment using sulfur-oxidizing bacteria, Thiobacillus sp. IW was studied. The colony size of UIW-10 was found 2 $^{\sim}$ 3 times bigger in diameter than the parent colony on TAM medium. UIW-10 mutant growth was two times higher than parent strain at 6 h culture in liquid medium containing sulfides such as sulfur and sodium thiosulfate. Initial pH and temperature for the optimum growth of UIW-10 were 6.0 and $35-40^{\circ}C$, respectively. It was found that addition of 0.5% yeast extract and 0.5 to 2.0% tryptone as nitrogen sources and the constant agitation at 150 to 200 rpm had a positive effect and the growth of UIW-10 was increased.

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