한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cloning and developing mutants of E.coli BL21(DE)/CrdS-F and E.coli BL21(DE)/CrdS-C for producing soluble glucan

  • Yin, Chun-Ji (Department of Chemical Engineering, Chosun University) ;
  • Min, Kyoung-Du (Department of Chemical Engineering, Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical Engineering, Chosun University)
  • Published : 2005.10.27
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Abstract

Water-soluble glucan was produced by mutants of E. coli BL21(DE)/CrdS-F and E. coli BL21(DE)/CrdS-C in a fermentor. Mutants of E. coli BL21(DE)/CrdS-F which has putative ${\beta}-1,3-glucan$ synthase catalytic subunit (gi:40556679) gene and E. coli BL21(DE)/CrdS-C which contains the active catalytic domain of partial curdlan synthase gene. The molecular weight of water-soluble glucan was analysed with HPLC.

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