Proceedings of the Polymer Society of Korea Conference (한국고분자학회:학술대회논문집)

The Polymer Society of Korea (한국고분자학회)
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X-ray Scattering Studies on the Structure of Porcine Pepsin in Various pH Solutions

  • Jin, Kyeong-Sik (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech)) ;
  • Jin, Sang-Woo (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech)) ;
  • Yoon, Jin-Hwan (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech)) ;
  • Heo, Kyu-Young (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech)) ;
  • Kim, Jae-Han (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech)) ;
  • Kim, Hee-Soo (Department of Microbiology, Dongguk University College of Medicine) ;
  • Ree, Moon-Hor (Department of Chemistry, National Research Lab for Polymer Synthesis & Physics, Pohang Accelerator Laboratory, Center for Integrated Molecular Systems, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology (Postech))
  • Published : 2006.10.13
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Abstract

In the present study, porcine pepsin in various pH conditions was investigated by small-angle X-ray scattering (SAXS) in order to detailed information on the structure and its variations with pH conditions. These analyses identified the structure of pepsin, and compared with that obtained by single crystallography. Moreover, this study found the structure' variations with changing pH conditions. All the results will be discussed with considering the conformational characteristics of pepsin in solution and further correlate to the biological and spectroscopic characteristics reported previously.

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