DETERMINATION OF AMINO ACIDS IN RICE(PALDAL) BY IONEXCHANGE CHROMATOGRAPHY

  • Lee, Chun-Yung (College of Agriculture, Seoul National University) ;
  • Lee, Tae-Yung (College of Agriculture, Seoul National University) ;
  • Kwon, Tai-Wan (College of Agriculture, Seoul National University)
  • Published : 1961.12.31

Abstract

The amino acid analysis of ground rice and the crude protein isolated from rice were performed by the most recent method of Moore, Spackman and Stein, using Amberlite IR-120. The effect of carbohydrates on the hydrolysis of rice was found to be definite in losing several amino acids nevertheless Dustin's claim that large excess of hydrolyzing acid reduces the losses of amino acid. Consequantly the removal carbohydrates as much as possible is considered to facilitate the amino acid determination. The amino acid composition of the crude rotein of rice was very similar to that previously found by other investigators.

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