Studies on Preparation of a Cheese-like product from Soybean Milk

콩을 이용한 치-즈제조에 관한 연구

1) Among five lactic acid bacteria examined, Str. thermophilus and Str. diacetilactis produced remarkably greater amount of acids in soybean milk than Str. lactis, Str. cremoris and L. bulgaricus. 2) Soybean milk and skimmed dry milk were combined in the ratio of 7 : 3 and were carried out in lactic acid fermentation for 24 hours at optimum temperature. The result indicated that the yield of precipitation and protein content of it were the most, the moisture content was the least and curd structure formed was considered too hard. 3) Based on these and other results, following procedure was used for manufacturing: soybean milk and skimmed dry milk were combined in the ratio of 7 : 3, heated at $121^{\circ}C$ for 20 min., cooled, added Str. thermophilus as lactic acid starter and incubated for 24 hours and $37{\pm}1^{\circ}C$. The curd was cooked, hooped, and pressed for 24 hours, to the surface of which, Penicillium caseicolum and sodium chloride were spread. During ripening of the curd at $15^{\circ}C$ and $85{\sim}90%$ RH for 21 days, Pen. caseicolum was highly developed after 7 days, pH was increased and proteolytie activity has reached to the peak point after 14 days. After 7 days of ripening total water soluble nitrogen, water soluble protein nitrogen and amino acids nitrogen were begun to increase. After 21 days of ripening total water soluble nitrogen, water soluble protein nitrogen and amino-N reached to 52%, 32% and 14% of total nitogen. In the soybean cheese, after 21 days of ripening, 17 or more kinds of amino acids were detected by two-dimentional paper chromatography. The product contained 63.2% of moisture, 17.5% of crude protein, 13.2% of crude fat, 2.8% of crude ash and 2.5% of sodium chloride.

1. 5섯가지 젖산균을 대두유에 접종배양 하여 산 생성도를 비교 하였드니 Str. thermophilus 와 Str. diacetilactis가 Str. lactis, Str. cremoris 및 L. bulgaricus보다 높았다. 2. 대두유와 탈지 분유를 7 : 3의 비로 혼합하여 젖산균 발효를 하였을 때에 침전물의 수획량이 가장 많고 또한 그의 단백질 함량이 가장 많으며 수분의 량이 적고 견고한 curd를 만들수 있음을 알았다. 3. 대두유와 탈지분유를 7 : 3의 비로 혼합한 것에 젖산균 starter로 Str. thermophilus를 첨가하고 Pen. caseicolum을 접종하여 제조한 치-즈를 3주간 숙성$(15^{\circ}C,\;RH:\;85{\sim}90%)$ 하였드니 숙성 7일째부터 Pen. caseicolum의 생육이 왕성하게 되며 pH가 상승함과 동시에 proteolytic activity는 14일째에 최고치에 도달하였다, 또 total water soluble nitrogen, water soluble protein nitrogen 및 amino-N도 숙성 7일째부터 증가하기 시작하여 숙성 21일째는 이들의 값이 각각 total nitrogen의 52%, 32% 및 14%에 이르렀다. 그리고 이차원 paper chromatography에 의하여 3주간 숙성한 치-즈에서 17종의 아미노산을 검출 하였다. 또 3주간 숙성한 후 제품의 일반성분 조성은 수분 63.2%, 조단백질 17.5%, 조지방 13.2%, 조회분 2.8% 및 식염 2.5%였다.