Studies on Cellulolytic Enzymes produced by Aspergillus saitoi -(1) Studies on the endocarp remove canning process of mandarine orange syrup-

Aspergillus Saitoi가 생산(生産)하는 섬유소분해효소(纖維素分解酵素)에 관(關)한 연구(硏究) -(1 보(1 報)) 온주밀감 통조림 제조시(製造時) 내과피(內果皮) 분해제거(分解除去)에 관(關)한 연구(硏究)-

To research were procedure condition of cellulase and remove effect of endocarp of mandarine orange from the result obtained it could be summarize as follow. 1. Optimal conditions affecting the production of cellulolytic enzyme of Aspergillus saitoi are followings: optimal temperature $30^{\circ}C$ and optimal culture period 3days. 2. When mandarine orange was soaked in 10 fold diluent of enzyme(diluent of enzyme: mandarine orange=2 : 1) and reacted under $40^{\circ}C$ for 3.5-4hrs, it was most effective and available to the remove of endocarp, flavour, and lustre important to the canning process of mandarine orange syrup.

Aspergillus saitoi가 생산(生産)하는 섬유소분해효소(纖維素分解酵素)의 생산조건(生産條件)과 밀감내과피(內果皮)의 탈피효과(脫皮效果)를 검토한 결과(結果)는 다음과 같다. 1. Asp. saitoi의 배양최적(培養最適) 온도(溫度)는 $30^{\circ}C$, 시간(時間)은 72시간(時間)에서 섬유소(纖維素) 생성능(生成能)이 가장 좋았다. 2. 본효소(本酵素)를 10배(倍) 농도로 희석하여 (희석효소액 : 밀감 2 : 1) $40^{\circ}C$의 항온조(恒溫槽)에서 $3.5{\sim}4$시간(時間) 작용(作用)시킨 것이 탈피정도(脫皮程度), 풍미(風味), 광택(光澤)이 우수하여 밀감 syrup 통조림 제조조건으로 최적(最適)이었다.