Korean Journal of Microbiology (미생물학회지)
- Volume 10 Issue 3
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- Pages.109-116
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- 1972
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Studies on Production of Nucieic acid Derivatives by Microorganisms(II)
미생물에 의한 핵산관련물질의 생산에 관한 연구 2
- Bae, Moo (Applid Microbiology Lab., Korea Institute of Science and Technology) ;
- Lee, Kye, Joon (Applid Microbiology Lab., Korea Institute of Science and Technology)
- Published : 1972.09.01
Abstract
Three strains among 120 adenineless mutants of Brevibacterium ammoniagenes described in the previous paper were screened out to accumulate UV absorbing substances in the culture broth. It was analyzed to be 5'-inosinic acid and hypoxanthine by means of two dimensional paper chromatography, UV absorption spectra and periodate oxidation. 5'-IMP was isolated from the culture broth of the mutant No. 203 with anion-exchange resin amberite IRA-400 and recrystallized from ethanol. It was proved identical to authentic sample by Infra-red absorption spectrum. The growth responses of the mutant No. 2013 were demonstrated to require adenine, but not with adenosine and 5'-AMP.
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