An Electron Microscopic Study on the Azurophil Granules Occurred in the Lymphocytes of the Chicken Peripheral Blood

닭순환임파구내에 출현하는 Azurophil 과립의 동태에 관한 전자현미경적 연구

With an effort to clarify the nature of the azurphil granules which occasionally occur in the circulating lymphocytes, these granules were investigated by examining smear of the peripheral blood of the chikens in various stage of the individual growth and after injection of methylene blue and gentian violet. In addition, the fine structure of these granules were also investigated. The results were: 1. These granules were first occurred in the lymphocytes just after their hatching (0.004%). The proportion of lymphocytes containing these granules were increased with their growth and in adult chicken its occurrence was higher than mammals. 2. Marked variations in its fine structure, particularly in its size and cotents, were noted but they were believed to belong to categories of lysosome of de Duve. 3. Lymphocytes containing azurophil granule were increased after injection of the non-immunogenic substances, such as gentian violet and methylene blue. 4. From the above results, chicken is bettor animal to study theme granules because of its higher occurrence. They are believed to have intimate relationship with bodily cellular reaction against the foreign materials because they are increased after non-immunogenic stimuli.