Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Studies on Separation, Detection and Quantitation of Estriol, Estrone, Estradiol-17 β in Urine of Dairy Cows by Paper, Thin Layer and Column Chromatography

Paper, Thin Layer 및 Column Chromatography에 의한 요중의 Estriol, Estrone, Estadiol-17 β의 분리 정량에 관하여

  • Yang, Yong Kwan (Department of Veterinary Medicine, College of Agriculture, Seoul National University) ;
  • Han, Soo Nam (Department of Veterinary Medicine, College of Agriculture, Seoul National University) ;
  • Cho, Jong Hoo (Institute of Veterinary Research, Office of Rural Development)
  • 양용관 (서울대학교 농과대학 수의학과) ;
  • 한수남 (서울대학교 농과대학 수의학과) ;
  • 조종후 (농촌진흥청 가축위생연구소)
  • Published : 1973.03.31
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Abstract

Thin layer, paper and column chromatography were compared for the separation, detection and quantitation of three kinds of estrogen in urine of dairy cows. While thin layer chromatography utilizing silica gel was better for the detection of estrogens, column chromatography using celite 545 was preferable. Spectrophotometry was compared with fluorometry for determination of estrone, estradiol-17 ${\beta}$ and estriol eluted by paper chromatography and column chromatography. Optical density of three standard estrogens showed almost same curve at maximum absorption wave length of 230 and $282m{\mu}$. However, the former showed a higher peak. In fluorometry, the fluorescence intensity of estrone and estradiol-17 ${\beta}$ were rather strong, when the estrogens were dissolved in sulfuric acid, and showed higher sensitivity than that of the spectrophotometry. However, in the case of estriol was exceptional.

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