Studies on the Constituents of Bordetella bronchiseptica. Purification and Properties of the Fractions Obtained by DEAE-cellulose Column Chromatography

Bordetella bronchiseptica의 균체성분(菌體成分)에 관한 연구(硏究) 특(特)히 DEAE-cellulose Chromatography에 의한 분획정제(分劃精制)에 대하여

An attempt was carried out to purify the cell components of Bordetella bronchiseptica by the mild procedures. From the supernatant of sonic treated colls, the K-agglutinogen, hemagglutinogen and skin necrotizing factor were separated by a successive use of DEAE-cellulose column chromatography and ammonium sulfate precipitation. The specific activity of purified K-agglutinogen was demonstrated by a gel-diffusion test and was also found to be free from other biologically active substances of B. bronchiseptica: namely hemagglutinin, skin-necrotizing factor and O-antigen.