국내(國內)의 변질미(變質米)에서 분리된 Aspergillus flavus 군(群)의 Aflatoxin 생성능(生成能)

Producibility of Aflatoxins by Aspergillus flavus Group Isolated from Deteriolated Rice in Korea

국내의 변질미(變質米)에서 분리 동정(同定)된 Aspergillus flavus 7균주의 aflatoxin 생성능(生成能)을 조사하기 위하여 쌀에 인위적으로 배양후 추출, 분리, 정량한 결과 모든 균주가 aflatoxin 특히 $B_1$을 많이 생성하였고 $G_1$은 생성하지 못하였다. 이들 균주의 aflatoxin 생성능(生成能)은 배양기의 변색과정 및 chloroform 추출액의 색깔과 밀접한 관계가 있었다. Aflatoxin을 가장 많이 생성한 균주는 A. flavus var. columnaris로서 쌀에서 최고 1ppm의 aflatoxin $B_1$을 생성하였으며 정제된 aflatoxin $B_1$은 자외선 흡수(吸收) 스펙트럼과 water 및 acetate adduct에 의한 유도체(誘導體)형성에 의하여 동정(同定)하였다.

In order to investigate the producibility of aflatoxins by seven Aspergillus flavus strains isolated from deteriolated rice in Korea, polished rice was artificially inoculated and subjected to isolation and quantitation of the mycotoxin. It was proved that all strains were capable of producing aflatoxins, preferentially $B_1$ but no $G_1$ at all and their producibility was closely related to the color of culture media and chloroform extracts. The strain producing the most aflatoxin was A. flavus var. columnaris, excreting 1 ppm on rice. Aflatoxin $B_1$ was isolated and identified by thin-layer chromatography, ultraviolet absorption spectra and derivative formation of water and acetate adducts.