Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Studies on the Myofibrillar Proteins Part 2. New Procedure for Extraction of Regulatory Proteins from Myofibrils

근원섬유단백질에 관한 연구 (제2보) 근수축 조절단백질의 새로운 정제방법

  • Yang, Ryung (Department of Food Engineering, College of Science and Engineering, Yon Sei University) ;
  • Kim, Chul-Jai (Department of Food Engineering, College of Science and Engineering, Yon Sei University) ;
  • Yu, Ju-Hyun (Department of Food Engineering, College of Science and Engineering, Yon Sei University) ;
  • Lee, Hyuk-Sin (Department of Food Engineering, College of Science and Engineering, Yon Sei University) ;
  • Cho, Young-Dong (Department of Biochemistry, College of Science and Engineering, Yon Sei University)
  • 양융 (연세대학교 이공대학 식품공학과) ;
  • 김철재 (연세대학교 이공대학 식품공학과) ;
  • 유주현 (연세대학교 이공대학 식품공학과) ;
  • 이혁신 (연세대학교 이공대학 식품공학과) ;
  • 조영동 (연세대학교 이공대학 생화학과)
  • Published : 1974.12.28
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Abstract

An attempt was made to study on new method for the extraction of the regulatory proteins from myofibrils, and the procedures for the preparation of desensitized actomyosin and for complete extraction of troponin-tropomyosin complex were developed. When myofibrils were treated through the procedures developed in this study, actomyosin obtained had no Ca-sensitivity, indicating that Ca-sensitizing protein factor had been removed completely from myofibril. Consequently, it was concluded that the procedures developed in this study were convenient to test whether Ca-sensitizing proteins has been removed or not. When Mg-activated ATPase activity of myofibril were measured, the myofibrillar ATPase turned into the actomyosin type ATPase with the progress of the treatment. This result was interpreted to show that the regulatory proteins of the myofibril seems to play a cementing role on the structure of myofibril. When supernatant containing the regulatory proteins were fractionated with $(NH_4)_2SO_4$ saturation solution, regulatory proteins, ${\alpha}-actinin$ and troponia-tropomyosin complex, could be obtained and they showed their typical phyoislogical activity which modify the actin-myosin interaction. The amount of troponin-tropomyosin complex in myofibril was 72 mg per g myofibril. This result was in good agreement with the results reported by many investigators, and therefore it was concluded that our procedures for the extraction of troponin-tropomyosin complex were desirable to study on the quantitative analysis of troponin-tropomyosin complex.

근육의 수축 및 사후강직은 myosin 과 actin 그리고 ATP 와의 상호작용에 의한 것임은 널리 알려진 정설(定說)이다. 최근 myosin 과 actin 및 ATP 의 상호작용이 근조절단백질의 지배를 받고 Ca ion 이 관여하고 있다는 것이 알려졌다. 그런데 이들 조절단백질은 수용성단백질로써의 성질을 가지고 있음에도 불구하고 염용성단백질 구분에 들어 있다. 본 연구의 목적은 염용성단백질 구분에 들어 있는 이들 조절단백질의 새로운 분리정제방법을 연구하는 데 있었다. 이를 위하여 본 연구에서는 새로운 정제방법의 flow sheet 를 작성하였다. 이 제안된 새로운 정제방법은 근원섬유중의 조절단백질의 함량을 정량적으로 추적할 수 있는 장점을 가지고 있다는데 그 특색이 있다.

Keywords