초록
유산염배지(乳酸鹽培地)에서 배양(培養)한 Selenomonas ruminantium 균체(菌體)를 초음파처리후(超音波處理後) 추출(抽出)한 효소(酵素)와 cofactor로서 ATP, CoA, $Mg^{++}$, UTP를 사용(使用)하여 in vitro에서 당지질(糖脂質)을 생합성(生合成)하여 본 결과(結果)는 다음과 같다. 1. Selenomonas ruminantium의 효소(酵素)에 의(依)한 $^{14}C-glucosamine$과 tridecyl-Co A로 부터의 당지질(糖脂質)의 in vitro 생합성(生合成)은 ATP, $Mg^{++}$, UTP,에 의(依)하여 촉진(促進)되었다. 2. 효소추출액(酵素抽出液)을 원심분리(遠心分離)로 분획(分劃)하고 각분획(各分劃)의 glycolipid 합성능(合成能)을 본 결과(結果) 105,000의 supernatant에 6,000rpm supernatant의 약(約) 2배(倍)의 효소활성(酵素活性)을 나타내는 조효소(粗酵素)가 존재(存在)함을 알았다. 3. 효소반응(酵素反應)은 30분(分)일 때 대부분(大部分)이 진행(進行)되며 1시간(時間)에서 거의 완결(完結)됨을 알았다.
Biosynthesis of glycolipid from glucosamine and $C_{13:0}$ fatty acid was attempted using an enzyme preparation which was extracted from Selenomonas ruminantium grown in lactic acid medium by means of ultrasonication, and with cofactors of ATP, Co A, $Mg^{++}$, and UTP. The results are summarized as follows: 1. The rate of synthesis of glycolipid from $^{14}C-glucosamine$ and tridecyl CoA by an enzyme from Selenomonas ruminantium was promoted by the presence of co-factors, ATP, Mg, and UTP. 2. The rate of incorporation of $^{14}C-glucosamine$ into glycolipid by the centrifugal fraction of bacterial preparation was the highest in the 105,000 g supernatant fraction, indicating about twice the enzyme activity as the 6,000 rpm supernatant fraction. 3. The biosynthesis of glycolipid from $^{14}C-glucosamine$ and tridecyl-CoA by the crude enzyme of the 105,000 g supernatant fraction of Selenomonas ruminantium cell preparation proceeded the most part of it in 30 minutes and completed in an hour.