Immunological Identification ofFferrobacillus ferrooxidans and Thiobacillus thiooxidans

Ferrobaillus ferrooxidans와 Tthiobacillus thiooxdans의 면역학적 동정연구

The chemolithoautotrophic bacteria, Freeobacillus ferroxidans and Thiobacillus thiooxidans were identified according to their immunological and serological properties. The antibody to these organisms was easily elicited in experimental animals, however, the overall serologicl reactivities were low according to different kinds of titration methods. By means of the quantitive and qualitative analysis such as hemagglutination or ouchterlony tests, F.ferroxidans and T. thiooxidans were different in their immunoreactivities, whereas the strains among the F.ferrooxidans were possessed, in some extent, the sharing antigenic determinants. In the results of the polyacrylamide gel electrophoresis/radioimmunometric method, the major antigenic determinants of the organisms illustrated the type specificitles in the fraction number of 20-30 in their gel electrophoretograms with some modifications of the antigenic moieties.